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Cu2+-modified hollow carbon nanospheres: an unusual nanozyme with enhanced peroxidase-like activity

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Abstract

A Cu2+-modified carboxylated hollow carbon nanospheres (Cu2+-HCNSs-COOH) was designed with enhanced peroxidase-like activity for the detection of hydrogen peroxide (H2O2) and degradation of methylene blue (MB). Hollow polymer nanospheres were fabricated from aniline, pyrrole, Triton-100, and ammonium persulfate via confined interfacial copolymerization reaction, which can be pyrolyzed to create HCNSs with the hollow gap diameter of about 20 nm under high temperature. Combining the synergistic effect of coordination and electrostatic interaction, Cu2+-HCNSs-COOH was constructed by anchoring Cu2+ on the surface of HCNSs-COOH. Furthermore, Cu2+-HCNSs-COOH has higher affinity for 3,3′,5,5′-tetramethylbenzidine and H2O2 of 0.20 mM and 0.88 mM, respectively. Based on the rapid response of Cu2+-HCNSs-COOH to H2O2, we constructed a colorimetric sensing platform by detecting the absorbance of the 3,3′,5,5′-tetramethylbenzidine-H2O2 system at 652 nm for quantifying H2O2, which holds good linear relationship between 1 and 150 μM and has a detection limit of 0.61 μM. We also investigated the degradation of MB in the presence of Cu2+-HCNSs-COOH and H2O2, which can degrade 80.7% pollutants within 30 min. This research developed an unusual nanozyme for bioassays and water pollution treatment, which broadened the way for the rapid development of clinical diagnostics and water pollution treatment.

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Funding

This work was partly financially supported by the National Natural Science Foundation of China (Nos. 21775033, 22076041) and the Open Project Funding of the State Key Laboratory of Biocatalysis and Enzyme Engineering (SKLBEE2019016).

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Authors and Affiliations

  1. State Key Laboratory of Biocatalysis and Enzyme Engineering, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, People’s Republic of China

    Junlun Zhu, Guan Luo, **aoxue **, Yijia Wang, Wei Wen, **uhua Zhang & Shengfu Wang

  2. State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, 430062, People’s Republic of China

    Jonathan Nimal Selvaraj

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  1. Junlun Zhu
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  2. Guan Luo
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  3. **aoxue **
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  4. Yijia Wang
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  5. Jonathan Nimal Selvaraj
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  6. Wei Wen
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  7. **uhua Zhang
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Correspondence to Wei Wen or Shengfu Wang.

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Zhu, J., Luo, G., **, X. et al. Cu2+-modified hollow carbon nanospheres: an unusual nanozyme with enhanced peroxidase-like activity. Microchim Acta 188, 8 (2021). https://doi.org/10.1007/s00604-020-04690-0

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