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A porous graphene sorbent coated with titanium(IV)-functionalized polydopamine for selective lab-in-syringe extraction of phosphoproteins and phosphopeptides

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Abstract

A novel polydopamine coated three-dimensional porous graphene aerogel sorbent carrying immobilized titanium(IV) ions (denoted as Ti4+@PDA@GA) was fabricated without using an organic solvent. The material is shown to be a viable carbon foam type of monolithic sorbent for selective lab-in-syringe enrichment of phosphoproteins and phosphopeptides. The phosphoproteins can be separated from a sample by aspiration and then bind to the sorbent. The analytes then can be dispensed within 5 min. The weight percent of titanium in the monolith typically is 14%, and the absorption capacities for the model proteins β-casein and κ-casein are 1300 and 1345 mg g−1, respectively. The absorption capacities for nonphosphoproteins are much smaller, typically 160 mg g−1 for β-lactoglobulin, 125 mg g−1 for bovine serum, and 4.8 mg g−1 for lysozyme. The results demonstrate that the selectivity for phosphoproteins was excellent on multiple biological samples including standard protein mixtures, spiked human blood serum, and drinking milk. The selective enrichment of phosphopeptides also makes the method a promising tool in phosphoproteomics.

Schematic of a polydopamine coated three-dimensional porous graphene aerogel for immobilization of titanium(IV) ions. The material served as a monolithic sorbent for selective enrichment of phosphopeptides and phosphoproteins from biological samples. The enrichment process can be carried out conveniently using a lab-in-syringe way.

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Acknowledgements

This work was supported by the Natural Science Foundation of China [grant numbers 21575076, 21621003]; the National Key Research and Development Program of China [grant numbers 2016YFA0203101,2017YFC0906902]; and the Bei**g Municipality Science and Technology Program [grant numbers D161100002116001].

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Authors and Affiliations

  1. MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology;Department of Chemistry, Tsinghua University, Bei**g, 100084, China

    Siyuan Tan, Jundong Wang, Qiang Han, Qionglin Liang & Mingyu Ding

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  1. Siyuan Tan
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  2. Jundong Wang
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  4. Qionglin Liang
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Correspondence to Qionglin Liang or Mingyu Ding.

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Tan, S., Wang, J., Han, Q. et al. A porous graphene sorbent coated with titanium(IV)-functionalized polydopamine for selective lab-in-syringe extraction of phosphoproteins and phosphopeptides. Microchim Acta 185, 316 (2018). https://doi.org/10.1007/s00604-018-2846-y

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