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A highly sensitive dual-readout assay based on gold nanoclusters for folic acid detection

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Abstracts

We describe a sensitive fluorometric and colorimetric dual-readout probe for folic acid (FA). It is based on the use of the gold nanoclusters (AuNCs) and cysteamine–modified gold nanoparticles (cyst-AuNPs). The bovine serum albumin stabilized AuNCs exhibit strong fluorescence emission at 652 nm. Upon addition of cyst-AuNPs, the fluorescence intensity of the AuNCs showed dramatic decrease due to the surface plasmon enhanced energy transfer process. This is due to an FA-induced aggregation of the cyst-AuNPs which shifts the absorption peaks from 530 to 670 nm. Thus, the surface plasmon enhanced energy transfer between cyst-AuNPs and AuNCs is weakened and the fluorescence intensity of AuNCs is recovered. The fluorescence intensity of the AuNCs/cyst-AuNPs system is proportional to the concentration of FA in the range from 0.11 to 2.27 μmol L−1. The dual-readout probe reported here was successfully applied to the determination of FA in spiked serum samples and folic acid tablets.

A novel sensitive and selective fluorometric and colorimetric dual functional probe is developed for folic acid detection.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (Nos. 21075050, No. 21275063) and the Science and Technology Development Project of Jilin Province, China (No. 20110334).

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Authors and Affiliations

  1. Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China

    Xu Yan, Bocheng Cao, Zeyang Ding & **ngguang Su

  2. School of Pharmacy, Jilin University, Changchun, 130021, China

    Hongxia Li

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  1. Xu Yan
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  2. Hongxia Li
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  3. Bocheng Cao
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  4. Zeyang Ding
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  5. **ngguang Su
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Correspondence to **ngguang Su.

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Yan, X., Li, H., Cao, B. et al. A highly sensitive dual-readout assay based on gold nanoclusters for folic acid detection. Microchim Acta 182, 1281–1288 (2015). https://doi.org/10.1007/s00604-014-1442-z

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  • DOI: https://doi.org/10.1007/s00604-014-1442-z

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