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Characterization of pyrene and chrysene degradation by halophilic Hortaea sp. B15

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Abstract

Polycyclic aromatics hydrocarbons (PAHs) are ubiquitous and toxic pollutants that are dangerous to humans and living organism in aquatic environment. Normally, PAHs has lower molecular weight such as phenanthrene and naphthalene that are easy and efficient to degrade, but high-molecular-weight PAHs such as chrysene and pyrene are difficult to be biodegraded by common microorganism. This study investigated the isolation and characterization of a potential halophilic bacterium capable of utilizing two high-molecular-weight PAHs. At the end of the experiment (25–30 days of incubation), bacterial counts have reached a maximum level (over 40 × 1016 CFU/mL). The highest biodegradation rate of 77% of chrysene in 20 days and 92% of pyrene in 25 days was obtained at pH 7, temperature 25 °C, agitation of 150 rpm and Tween 80 surfactant showing to be the most impressive parameters for HMWPAHs biodegradation in this research. The metabolism of initial compounds revealed that Hortaea sp. B15 utilized pyrene to form phthalic acid while chrysene was metabolized to form 1-hydroxy-2-naphthoic acid. The result showed that Hortaea sp. B15 can be promoted for the study of in situ biodegradation of high molecular weight PAH.

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Acknowledgements

The authors would like to thank the Deanship of Scientific Research at King Saud University for funding this work through research group no. RG1439-044, Malaysian Ministry of Higher Education through Fundamental Research Grant Scheme no. 5F244.

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Authors and Affiliations

  1. Botany and Microbiology Department, College of Sciences, King Saud University, P.O. Box 22452, Riyadh, 11495, Saudi Arabia

    Dunia A. Al Farraj, Mohamed Soliman Elshikh, Manal M. Al Khulaifi & Roua Al-Kufaidy

  2. Department of Environmental Engineering, Faculty of Engineering and Science, Curtin University, Malaysia, CDT 250, 98009, Miri, Sarawak, Malaysia

    Tony Hadibarata

  3. Department of Environmental Engineering, Faculty of Civil, Environmental and Geo Engineering, Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia

    Adhi Yuniarto

  4. Department of Water and Environmental Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310, Johor Bahru, Malaysia

    Achmad Syafiuddin

  5. Biology Program, Faculty of Educational Science and Mathematics, Universitas Pendidikan Indonesia, Jl Dr. Setiabudi No. 229, Bandung, Indonesia

    Hertien Koosbandiah Surtikanti

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  1. Dunia A. Al Farraj
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  2. Tony Hadibarata
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Correspondence to Dunia A. Al Farraj.

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Al Farraj, D.A., Hadibarata, T., Yuniarto, A. et al. Characterization of pyrene and chrysene degradation by halophilic Hortaea sp. B15. Bioprocess Biosyst Eng 42, 963–969 (2019). https://doi.org/10.1007/s00449-019-02096-8

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  • DOI: https://doi.org/10.1007/s00449-019-02096-8

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