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Peptide immunocytochemistry of neurons projecting to the retrocerebral complex in the blow fly, Protophormia terraenovae

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Abstract

Antisera against a variety of vertebrate and invertebrate neuropeptides were used to characterize neurons with somata in the pars intercerebralis (PI), pars lateralis (PL), and subesophageal ganglion (SEG), designated as PI neurons, PL neurons, and SEG neurons, respectively, all of which project to the retrocerebral complex in the blow fly, Protophormia terraenovae. Immunocytochemistry combined with backfills through the cardiac-recurrent nerve revealed that at least two pairs of PI and SEG neurons for each were FMRFamide-immunoreactive. Immunoreactivity against [Arg7]-corazonin, β-pigment-dispersing hormone (β-PDH), cholecystokinin8, or FMRFamide was observed in PL neurons. Immunoreactive colocalization of [Arg7]-corazonin with β-PDH, [Arg7]-corazonin with cholecystokinin8, or β-PDH with FMRFamide was found in two to three somata in the PL of a hemisphere. Based on their anatomical and immunocytochemical characteristics, PI neurons were classified into two types, PL neurons into six types, and SEG neurons into two types. Fibers in the retrocerebral complex showed [Arg7]-corazonin, β-PDH, cholecystokinin8, and FMRFamide immunoreactivity. Cholecystokinin8 immunoreactivity was also detected in intrinsic cells of the corpus cardiacum. The corpus allatum was densely innervated by FMRFamide-immunoreactive varicose fibers. These results suggest that PI, PL, and SEG neurons release [Arg7]-corazonin, β-PDH, cholecystokinin8, or FMRFamide-like peptides from the corpus cardiacum or corpus allatum into the hemolymph, and that some PL neurons may simultaneously release several neuropeptides.

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Acknowledgements

We are grateful to Dr. N.T. Davis (University of Arizona) for reading through the manuscript and giving critical comments and for a gift of antisera. We also thank Dr. J. A. Veenstra (Université Bordeaux), Dr. M.R. Brown (University of Georgia), Dr. H. Dircksen (University of Bonn), Dr. A. Mizoguchi (Nagoya University), Dr. D.R. Nässel (Stockholm University), and Dr. B. Stay (University of Iowa) for their generous gifts of antisera.

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  1. Department of Biology and Geosciences, Graduate School of Science, Osaka City University, Sumiyoshi, Osaka, 558-8585, Japan

    Yoshitaka Hamanaka, Shinji Tanaka, Hideharu Numata & Sakiko Shiga

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  1. Yoshitaka Hamanaka
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  2. Shinji Tanaka
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  3. Hideharu Numata
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  4. Sakiko Shiga
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Correspondence to Sakiko Shiga.

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This work was supported by a Grant-in-Aid for Scientific Research C (17570065) from the Japan Society for the Promotion of Science (to S.S.) and a Grant-in-Aid for Scientific Research B (16370038) from the Japan Society for the Promotion of Science (to H.N.).

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Hamanaka, Y., Tanaka, S., Numata, H. et al. Peptide immunocytochemistry of neurons projecting to the retrocerebral complex in the blow fly, Protophormia terraenovae . Cell Tissue Res 329, 581–593 (2007). https://doi.org/10.1007/s00441-007-0433-3

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