Abstract
The barley Rar1 gene is an essential component of the race-specific, Mla-12-specified powdery mildew resistance reaction. As part of a map-based cloning strategy designed to isolate Rar1, five barley yeast artificial chromosomes (YACs) have been identified, ranging in size from 300 to 1100 kb. PCR-based YAC end-specific markers have been established and were employed to construct a local YAC contig. Four out of five YAC clones were found to be non-colinear with the source DNA. High-resolution genetic map** of the YAC ends demonstrated that the set of five overlap** YAC clones encompasses the barley Rar1 gene.
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Received: 9 June 1998 / Accepted: 15 July 1998
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Lahaye, T., Shirasu, K. & Schulze-Lefert, P. Chromosome landing at the barley Rar1 locus. Mol Gen Genet 260, 92–101 (1998). https://doi.org/10.1007/s004380050874
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DOI: https://doi.org/10.1007/s004380050874