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Genome wide identification of C1-2i zinc finger proteins and their response to abiotic stress in hexaploid wheat

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Abstract

The C1-2i wheat Q-type C2H2 zinc finger protein (ZFP) transcription factor subclass has been reported to play important roles in plant stress responses. This subclass of ZFPs has not been studied in hexaploid wheat (Triticum aestivum) and we aimed to identify all members of this subclass and evaluate their responses to different abiotic stresses causing oxidative stress. Exploiting the recently published wheat draft genome sequence, we identified 53 members (including homoeologs from A, B and D genomes) of the C1-2i wheat Q-type C2H2 ZFPs (TaZFPs) representing 21 genes. Evolution analysis revealed that 9 TaZFPs members are directly inherited from the parents Triticum urartu and Aegilops tauschii, while 15 diverged through neoploidization events. This TaZFP subclass is responsive to the oxidative stress generator H2O2 and to high light, drought stress and flooding. Most TaZFPs are responsive to H2O2 (37/53), high light (44/53), flooding (31/53) or drought (37/53); 32 TaZFPs were up-regulated by at least 3 stresses and 16 were responsive to all stresses tested. A large number of these TaZFPs were physically mapped on different wheat draft genome sequences with known markers useful for QTL map**. Our results show that the C1-2i subclass of TaZFPs is associated with responses to different abiotic stresses and that most TaZFPs (30/53 or 57 %) are located on group 5 chromosomes known to be involved in environment adaptation. Detailed characterization of these novel wheat TaZFPs and their association to QTL or eQTL may help to design wheat cultivars with improved tolerance to abiotic stress.

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Acknowledgments

The authors thank Dr. F. Ouellet and Dr. F. Sarhan for critical reading of the manuscript. They also thank Dr. F. Ouellet for sharing the CFX96 TouchTM Thermal cycler (Bio-Rad).

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  1. Département des sciences biologiques, Centre TOXEN, Université du Québec à Montréal, Montreal, QC, H3C 3P8, Canada

    Arnaud Cheuk & Mario Houde

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  1. Arnaud Cheuk
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Correspondence to Mario Houde.

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A.C. acknowledges support from the Faculty of Science at UQAM for a Ph. D. scholarship and for a scholarship exempting international student tuition fees. This work was supported by a Natural Sciences and Engineering Research Council of Canada grant (OGP0138557) to M.H.

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Cheuk, A., Houde, M. Genome wide identification of C1-2i zinc finger proteins and their response to abiotic stress in hexaploid wheat. Mol Genet Genomics 291, 873–890 (2016). https://doi.org/10.1007/s00438-015-1152-1

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