Abstract
Southern Corn Leaf Blight (SCLB) is an important disease in warm-temperate and tropical corn-producing areas throughout the world. We applied a combination of the amplified fragment length polymorphism (AFLP) technique and bulked segregant analysis (BSA) to a large F2 population in order to identify molecular markers linked to the rhm gene for resistance to SCLB. One co-dominant AFLP marker, p7m36, was mapped to a position 1.0 cM from rhm, and we converted this marker to an STS (sequence-tagged site) marker. Combined with the previously identified agrP144, this new marker may be useful for map-based cloning of the rhm gene and marker-assisted selection for rhm.
Similar content being viewed by others
References
Craig J, Fajemisin JM (1969) Inheritance of chlorotic lesion resistance to Helminthosporium maydis in maize. Plant Dis Rep 53:742–743
Hooker AL (1972) Southern leaf blight of corn—present status and future prospects. J Environ Qual 1:244–249
Hooker AL, Smith DR, Lim SM, Musson MD (1970) Physiological races of Helminthosporium maydis and disease resistance. Plant Dis Rep 54:1109–1110
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer program for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
McClintock B (1929) Chromosome morphology in Zea mays. Science 69:629
McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular map** of rice chromosomes. Theor Appl Genet 76:815–829
Meksem K, Leister D, Paleman J, Zabeau M, Salamini F, Gebhardt C (1995) A high-resolution map of the vicinity of the R1 locus on chromosome V of potato based on RFLP and AFLP markers. Mol Gen Genet 249:74–81
Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genome regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832
Myburg AA, Remington DL, O'Malley DM, Sederoff RR, Whetten RW (2001) High-throughput AFLP analysis using infrared dye-labeled primers and an automated DNA sequencer. BioTechniques 30:348–357
Ouedraogo JT, Maheshwari V, Berner DK, St Oierre C-A, Belzile F, Timko MP (2001) Identification of AFLP markers linked to resistance of cowpea ( Vigna unguiculata L.) to parasitism by Striga gesnerioides. Theor Appl Genet 102:1029–1036
Pate JB, Harvey PH (1954) Studies on the inheritance of resistance in corn to Helminthosporium maydis leaf spot. Agron J 46:442–445
Simons G, van der Lee T, Diergaarde P, van Daelen R, Groendijk J, Frijters A, Buschges R, Hollricher K, Topsch S, Schulze-Lefert P, Salamini F, Zabeau M, Vos P (1997) AFLP-based fine map** of the Mlo gene to a 30-kb DNA segment of the barley genome. Genomics 44:61–70
Smith DR (1975) expression of monogenic chlorotic-lesion resistance to Helminthosporium maydis in corn. Phytopathology 65:1160–1165
Smith DR, Hooker AL (1973) Monogenic chorotic-lesion resistance in corn to Helminthosporium maydis. Crop Sci 13:330–331
Tai T, Dahlbeck D, Stall RE, Peleman J, Staskawicz BJ (1999) High-resolution genetic and physical map** of the region containing the Bs2resistance gene of pepper. Theor Appl Genet 99:1201–1206
Thompson DL, Berquist RR (1984) Inheritance of mature plant resistance to Helminthosporium maydis race O in maize. Crop Sci 24:807–811
Ullstrup AJ (1970) History of southern corn leaf blight. Plant Dis Rep 54:1100–1102
Ullstrup AJ (1978) Evolution and dynamics of corn diseases and insect problems since the advent of hybrid corn. In: Walden DB (ed) Maize breeding and genetics. Wiley-Interscience, New York, pp 283–297
Von Malek B, Weber WE, Debener T (2000) Identification of molecular markers linked to Rdr1, a gene conferring resistance to blackspot in roses. Theor Appl Genet 101:977–983
Vos P, Hogers R, Bleekers M, Reijans M, van der Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414
Wei JK, Liu KM, Chen JP, Luo PC, Lee-Stadelmann OY (1988) Pathological and physiological identification of race O of Bipolaris maydis in China. Phytopathology 78:550–554
Xu ML, Melchinger AE, ** of loci conferring resistance to sugarcane mosaic virus in maize using RFLP, SSR, and AFLP markers. Mol Gen Genet 261:574–581
Yang HY, Korban SS, Kruger J, Schmidt H (1997) The use of a modified bulk segregant analysis to identify a molecular marker linked to a scab resistance gene in apple. Euphytica 94:175–182
Zaitlin D, DeMars S, Ma Y (1993) Linkage of rhm, a recessive gene for resistance to southern corn leaf blight, to RFLP marker loci in maize ( Zea mays) seedlings. Genome 36:555–564
Acknowledgements
We thank Dr. H. Morishima (Tokyo University of Agriculture, Tokyo, Japan) and the two anonymous reviewers for their valuable comments on this manuscript. We also acknowledge Dr. A. A. Myburg (North Carolina State University, Raleigh, NC, USA) for his technical advice on AFLP analysis. This study was supported by a grant from the Japan Racing Association
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by R. Hagemann
Rights and permissions
About this article
Cite this article
Cai, Hw., Gao, Zs., Yuyama, N. et al. Identification of AFLP markers closely linked to the rhm gene for resistance to Southern Corn Leaf Blight in maize by using bulked segregant analysis. Mol Gen Genomics 269, 299–303 (2003). https://doi.org/10.1007/s00438-003-0837-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00438-003-0837-z