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Sporulation rate and viability of Eimeria tenella oocysts stored in potassium sorbate solution

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Abstract

In order to find a new preservation solution for avian coccidial oocysts that can replace potassium dichromate (K2Cr2O7) solution, Eimeria tenella oocysts were preserved in 0.1 to 10% potassium sorbate (C6H7KO2) solution in this study. The results showed that there was no significant difference between the sporulation rate of E. tenella oocysts preserved in 0.1 to 10% C6H7KO2 solution and in 2.5% K2Cr2O7 solution (p > 0.05). The 0.5 to 10% C6H7KO2 solution could also effectively inhibit the growth of bacterial microorganisms. E. tenella oocysts preserved in 1% C6H7KO2 solution at 4 °C for 3, 6, 9, and 12 months, with the oocyst production of E. tenella oocysts being 1.3-, 1.2-, 1.6-, and 1.3-fold higher than that of oocysts stored in 2.5% K2Cr2O7 solution (p < 0.05). In conclusion, C6H7KO2 could replace K2Cr2O7 as the preservation solution of avian coccidial oocysts.

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Data availability

The dataset supporting the conclusions of this article is included within the article.

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Funding

This research was supported by the National Key Research and Development Program of China (2017YFD0501200) and the Lanzhou major science and technology project (2017-2-2) to HY.

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Authors and Affiliations

  1. China Agricultural Vet. Bio. Science and Technology Co., Ltd., Gansu, 730046, Lanzhou, China

    Zhenxing Gong, Hongmei Wei, Fang Chang & Hong Yin

  2. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu, China

    Hong Yin & Jian** Cai

Authors
  1. Zhenxing Gong
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  2. Hongmei Wei
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  3. Fang Chang
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  4. Hong Yin
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  5. Jian** Cai
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Contributions

ZXG and HMW performed the experiments. ZXG analyzed the data. FC contributed reagents/materials. ZXG wrote the paper. JPC and HY designed the experiments and revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Jian** Cai.

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Gong, Z., Wei, H., Chang, F. et al. Sporulation rate and viability of Eimeria tenella oocysts stored in potassium sorbate solution. Parasitol Res 120, 2297–2301 (2021). https://doi.org/10.1007/s00436-020-06792-3

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  • DOI: https://doi.org/10.1007/s00436-020-06792-3

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