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Morphology and 18S rDNA sequencing identifies Henneguya visibilis n. sp., a parasite of Leporinus obtusidens from Mogi Guaçu River, Brazil

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Abstract

During a survey of myxozoan parasites of freshwater fish from the Mogi Guaçu River in São Paulo State, Brazil, plasmodia of Henneguya visibilis n. sp. were found on the fins of Leporinus obtusidens (Characiformes: Anostomidae). The plasmodia, which were observed on five out of eight (62.5 %) L. obtusidens examined, were 400–1,000 μm long. Mature spores were elongated with a spore body 10.8 ± 0.6 μm long and 3.9 ± 0.2 μm wide, a caudal process 18 ± 1.2 μm long, and a total spore length of 26.8 ± 1.1 μm. Polar capsules were elongated 4.9 ± 0.3 μm long and 1.4 ± 0.1 μm wide. Histological examination indicated that the plasmodia developed in the connective tissue, and no inflammatory infiltrate was observed at the infection site. Ultrastructural analysis showed a plasmodium wall with a single membrane and several pinocytotic canals. Sporogenesis occurred from the periphery to the center of the plasmodia. Phylogenetic analysis of the 18S rDNA sequence using maximum likelihood and maximum parsimony methods showed H. visibilis n. sp. positioned in a sub-clade composed of Henneguya/Myxobolus parasites of several freshwater fish families.

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Acknowledgments

This study was supported by the Brazilian fostering agency CNPq (grant number 477658/2010-5). The authors are grateful to the doctoral students Juliana Naldoni, Mateus M. Carriero, and Tiago Milanin for their help in laboratory analyses.

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Authors and Affiliations

  1. Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo (USP), Rua Duque de Caxias Norte, 225, Pirassununga, São Paulo, 13635-900, Brazil

    Gabriel S. A. Moreira, Marcia R. M. Silva & Antônio A. M. Maia

  2. Departamento de Ciências Biológicas, Universidade Federal de São Paulo (Unifesp), Rua Professor Artur Riedel, 275, Jardim Eldorado, Diadema, São Paulo, 09972-270, Brazil

    Edson A. Adriano

  3. Departamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Caixa Postal 6109, Campinas, São Paulo, 13083-970, Brazil

    Edson A. Adriano

  4. Centro Nacional de Pesquisa e Conservação de Peixes Continentais (CEPTA), Instituto Chico Mendes de Conservação da Biodiversidade (ICMBio), Rod. SP 201, Km 6.5, Caixa Postal 64, Pirassununga, São Paulo, 13630-970, Brazil

    Paulo S. Ceccarelli

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  1. Gabriel S. A. Moreira
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Correspondence to Edson A. Adriano.

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Moreira, G.S.A., Adriano, E.A., Silva, M.R.M. et al. Morphology and 18S rDNA sequencing identifies Henneguya visibilis n. sp., a parasite of Leporinus obtusidens from Mogi Guaçu River, Brazil. Parasitol Res 113, 81–90 (2014). https://doi.org/10.1007/s00436-013-3629-3

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