Abstract
The species of liver flukes of the genus Fasciola (Platyhelminthes: Digenea: Fasciolidae) are obligate parasitic trematodes residing in the large biliary ducts of herbivorous mammals. While Fasciola hepatica has a cosmopolitan distribution, the other major species, i.e., Fasciola gigantica is reportedly prevalent in the tropical and subtropical regions of Africa and Asia. To determine the phylogenic location of Fasciola sp. of Assam (India) origin based on rDNA molecular data, ribosomal ITS regions were sequenced and compared with other species of trematodes in the family Fasciolidae. NCBI databases were used for sequence homology analysis using BLAST and ClustalW programs. The phylogenetic trees constructed based upon the ITS (1 and 2) sequences revealed a close relationship with isolates of F. gigantica from China, Indonesia, Japan, Egypt, and Zambia, the isolate from China with significant bootstrap values being the closest. Using the novel approach of molecular morphometrics that is based on ITS2 secondary structure homologies, phylogenetic relationships of the various isolates of fasciolid species have also been discussed. While comparing ITS1, the sequence of another Indian isolate designated as F. gigantica (accession no. EF198867) showed almost absolute match with F. hepatica. Hence, this particular isolate should be identified as F. hepatica and not F. gigantica.
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Acknowledgements
This study was carried out under the “All India Co-ordinated Project on Capacity Building in Taxonomy: Research on Helminths,” sanctioned to VT by the Ministry of Environment and Forests, GOI; DBT Project to VT and AC; DSA (UGC-SAP) in Zoology and UGC’s UPE (Biosciences) program in the School of Life Sciences at NEHU, Shillong.
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The nucleotide sequence data reported in this paper have been submitted to the GenBank data with the accession numbers EF027103 and EF027104.
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Prasad, P.K., Tandon, V., Biswal, D.K. et al. Molecular identification of the Indian liver fluke, Fasciola (Trematoda: Fasciolidae) based on the ribosomal internal transcribed spacer regions. Parasitol Res 103, 1247–1255 (2008). https://doi.org/10.1007/s00436-008-1121-2
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DOI: https://doi.org/10.1007/s00436-008-1121-2