SpringerLink
Log in

Effect of variation in RANTES promoter on serum RANTES levels and risk of recurrent wheezing after RSV bronchiolitis in children from Han, Southern China

  • Original Paper
  • Published:
European Journal of Pediatrics Aims and scope Submit manuscript

Abstract

To investigate the association among RANTES (regulated on activation normal T cell expressed and secreted) gene promoter polymorphism, serum RANTES levels, and recurrent wheezing after RSV (respiratory syncytial virus) bronchiolitis in children (1–12 months of age) from Han, Southern china. Three hundred twenty children with RSV bronchiolitis and 272 controls were enrolled in the 3-year follow-up study. The polymerase chain reaction–restriction fragment length polymorphism (PCR-RELP), enzyme-linked immunosorbent assay (ELISA) kit and luciferase analysis were the mainly used methods, which were used to genotype the RANTES (−403G/A), assess the serum RANTES levels and the RANTES promoter activity. As the results showed, the RANTES (−403 G/A) in the promoter region was associated with recurrent wheezing after RSV bronchiolitis (p < 0.05) and serum RANTES levels (RANTES genotype G/G: 26.03 ± 7.46 ng/ml G/A: 28.22 ± 6.44 ng/ml A/A: 30.12 ± 5.88 ng/ml). Functional analyses of RANTES promoter activity indicated that the RANTES (−403 G to A) mutation increases the transcriptional activity of the RANTES promoter. In conclusion, the RANTES (−403 G/A) polymorphism increases RANTES transcriptional activity resulted in a high serum RANTES levels, thus increased the risk of recurrent wheezing after RSV bronchiolitis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

Abbreviations

RANTES:

regulated on activation normal T cell expressed and secreted

RSV:

respiratory syncytial virus

PCR-RELP:

polymerase chain reaction–restriction fragment length polymorphism

ELISA:

enzyme-linked immunosorbent assay

IRF:

interferon response factor

References

  1. Al-Abdulhadi SA, Helms PJ, Main M et al (2005) Preferential transmission and association of the −403 G → A promoter RANTES polymorphism with atopic asthma. Genes Immun 6:24–30

    PubMed  CAS  Google Scholar 

  2. Amanatidou V, Sourvinos G, Apostolakis S et al (2008) RANTES promoter gene polymorphisms and susceptibility to severe respiratory syncytial virus-induced bronchiolitis. The Pediatr Infect Dis J 27:38–42

    Article  Google Scholar 

  3. An P, Nelson GW, Wang L et al (2002) Modulating influence on HIV/AIDS by interacting RANTES gene variants. PNAS 99:10002–10007

    Article  PubMed  CAS  Google Scholar 

  4. Bont L, Heijnen CJ, Kavelaars A et al (2000) Monocyte IL-10 production during respiratory syncytial virus bronchiolitis is associated with recurrent wheezing in a one-year follow-up study. Am J Respir Crit Care Med 161:1518–1523

    PubMed  CAS  Google Scholar 

  5. Everard ML, Milner AD (1992) The respiratory syncytial virus and its role in acute bronchiolitis. Eur J Pediatr 151:638–651

    Article  PubMed  CAS  Google Scholar 

  6. Fryer AA, Spiteri MA, Bianco A et al (2000) The −403 G→A promoter polymorphism in the RANTES gene is associated with atopy and asthma. Genes Immun 1:509–514

    Article  PubMed  CAS  Google Scholar 

  7. Genin P, Algarte M, Roof P et al (2000) Regulation of RANTES chemokine gene expression requires cooperativity between NF-kappa B and IFN-regulatory factor transcription factors. J Immunol 164:5352–5361

    PubMed  CAS  Google Scholar 

  8. Hajeer AH, al Sharif F, Ollier WE (1999) A polymorphism at position −403 in the human RANTES promoter. Eur J Immunogenet 26:375–376

    Article  PubMed  CAS  Google Scholar 

  9. Hull J, Thomson A, Kwiatkowski D (2000) Association of respiratory syncytial virus bronchiolitis with the interleukin 8 gene region in UK families. Thorax 55:1023–1027

    Article  PubMed  CAS  Google Scholar 

  10. Ichinohe S, Hussain IR, Johnston SL (2003) Cytokine production of RSV/PHA-stimulated tonsillar mononuclear cells: influences of age and atopy. Eur Respir J 22:317–322

    Article  PubMed  CAS  Google Scholar 

  11. John AE, Berlin AA, Lukacs NW (2003) Respiratory syncytial virus-induced CCL5/RANTES contributes to exacerbation of allergic airway inflammation. Eur J Immunol 33:1677–1685

    Article  PubMed  CAS  Google Scholar 

  12. Kotaniemi-Syrjanen A, Reijonen TM, Korhonen K et al (2002) Wheezing requiring hospitalization in early childhood: predictive factors for asthma in a six-year follow-up. Pediatr Allergy Immunol 13:418–425

    Article  PubMed  Google Scholar 

  13. Liu H, Chao D, Nakayama EE et al (1999) Polymorphism in RANTES chemokine promoter affects HIV-1 disease progression. PNAS 96:4581–4585

    Article  PubMed  CAS  Google Scholar 

  14. Martinez FD (2003) Respiratory syncytial virus bronchiolitis and the pathogenesis of childhood asthma. The Pediatr Infect Dis J 22:S76–S82

    Article  Google Scholar 

  15. Martinez FD, Wright AL, Taussig LM et al (1995) Asthma and wheezing in the first six years of life. The Group Health Medical Associates. The New Engl J Med 332:133–138

    Article  CAS  Google Scholar 

  16. Meurer R, Van Riper G, Feeney W et al (1993) Formation of eosinophilic and monocytic intradermal inflammatory sites in the dog by injection of human RANTES but not human monocyte chemoattractant protein 1, human macrophage inflammatory protein 1 alpha, or human interleukin 8. The J Exp Med 178:1913–1921

    Article  CAS  Google Scholar 

  17. Murai H, Terada A, Mizuno M et al (2007) IL-10 and RANTES are elevated in nasopharyngeal secretions of children with respiratory syncytial virus infection. Allergol Int 56:157–163

    Article  PubMed  CAS  Google Scholar 

  18. Nickel RG, Casolaro V, Wahn U et al (2000) Atopic dermatitis is associated with a functional mutation in the promoter of the C–C chemokine RANTES. J Immunol 164:1612–1616

    PubMed  CAS  Google Scholar 

  19. Schaap T (1980) The applicability of the Hardy–Weinberg principle in the study of populations. Ann Hum Genet 44:211–215

    Article  PubMed  CAS  Google Scholar 

  20. Schall TJ (1991) Biology of the RANTES/SIS cytokine family. Cytokine 3:165–183

    Article  PubMed  CAS  Google Scholar 

  21. Schall TJ, Bacon K, Toy KJ et al (1990) Selective attraction of monocytes and T lymphocytes of the memory phenotype by cytokine RANTES. Nature 347:669–671

    Article  PubMed  CAS  Google Scholar 

  22. Sigurs N, Bjarnason R, Sigurbergsson F et al (2000) Respiratory syncytial virus bronchiolitis in infancy is an important risk factor for asthma and allergy at age 7. Am J Respir Crit Care Med 161:1501–1507

    PubMed  CAS  Google Scholar 

  23. Sigurs N, Gustafsson PM, Bjarnason R et al (2005) Severe respiratory syncytial virus bronchiolitis in infancy and asthma and allergy at age 13. Am J Respir Crit Care Med 171:137–141

    Article  PubMed  Google Scholar 

  24. Tekkanat KK, Maassab H, Miller A et al (2002) RANTES (CCL5) production during primary respiratory syncytial virus infection exacerbates airway disease. Eur J Immunol 32:3276–3284

    Article  PubMed  CAS  Google Scholar 

  25. Yao TC, Kuo ML, See LC et al (2003) The RANTES promoter polymorphism: a genetic risk factor for near-fatal asthma in Chinese children. The J Allergy Clin Immunol 111:1285–1292

    Article  CAS  Google Scholar 

Download references

Acknowledgment

This study was supported by Foundation of Nan**g Municipal Health Bureau (YKK05121).

Author information

Authors and Affiliations

  1. Department of Respiratory Medicine, Nan**g Children’s Hospital Affiliated to Nan**g Medical University, Nan**g, 210029, China

    Man Tian, Feng Liu, Guan-yu Wen & De-yu Zhao

  2. Institute of Pediatrics, Nan**g Children’s Hospital Affiliated to Nan**g Medical University, Nan**g, 210029, China

    Sheng-yun Shi

  3. Institute of Pediatrics of Nan**g Medical University, Nan**g, 210029, China

    Rong-hua Chen

Authors
  1. Man Tian
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Feng Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Guan-yu Wen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sheng-yun Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Rong-hua Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. De-yu Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to De-yu Zhao.

Additional information

Man Tian and Feng Liu contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tian, M., Liu, F., Wen, Gy. et al. Effect of variation in RANTES promoter on serum RANTES levels and risk of recurrent wheezing after RSV bronchiolitis in children from Han, Southern China. Eur J Pediatr 168, 963–967 (2009). https://doi.org/10.1007/s00431-008-0870-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00431-008-0870-3

Keywords

Search

Navigation