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Spatiotemporal expression of duplicate AGAMOUS orthologues during floral development in Phalaenopsis

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Abstract

The AGAMOUS (AG) family of MADS-box genes plays important roles in controlling the development of the reproductive organs of flowering plants. To understand the molecular mechanisms behind the floral development in the orchid, we isolated and characterized two AG-like genes from Phalaenopsis that we denoted PhalAG1 and PhalAG2. Phylogenetic analysis indicated that PhalAG1 and PhalAG2 fall into different phylogenetic positions in the AG gene family as they belong to the C- and D-lineages, respectively. Reverse transcription-polymerase chair reaction (RT-PCR) analyses showed that PhalAG1 and PhalAG2 transcripts were detected in flower buds but not in vegetative organs. Moreover, in situ hybridization experiments revealed that PhalAG1 and PhalAG2 hybridization signals were observed in the lip, column, and ovule during the floral development of Phalaenopsis, with little difference between the expression patterns of the two genes. These results suggest that both AG-like genes in Phalaenopsis act redundantly with each other in floral development.

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Acknowledgements

We deeply thank Professor H. Takahashi and S. Saito for technical assistance and also H. Tokairin for his collaboration in culturing the plants. We are also grateful to professor H. -Y. Lee, J. -H. Park, S. -S. Lee, Y. Ishikawa, T. Ochiai, P. -Y. Yun, B. -J. Park, Y. Mashiko, H. Ashizawa, A. Sato, M. Nakada, N. Kuroiwa, T. Shishido, R. Shinohara, M. Komatsu, Y. Akita, S. -Y. Kim, M. Hirai, T. Kamimura and H. Nakayama for providing much help and advice. This study was partly supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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  1. Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai, 980-8577, Japan

    In-Ja Song, Toru Nakamura, Tatsuya Fukuda, Takuro Ito, Toshiaki Kameya & Akira Kanno

  2. Graduate School of Life Sciences, Tohoku University, Aoba, Aoba-ku, Sendai, 980-8578, Japan

    Jun Yokoyama

  3. Akabira Orchid Nursery, Hyakko-cho Nishi 6-26, Akabira, 079-1273, Hokkaido, Japan

    Hiroaki Ichikawa

  4. Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, 080-8555, Hokkaido, Japan

    Yoh Horikawa

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  1. In-Ja Song
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Song, IJ., Nakamura, T., Fukuda, T. et al. Spatiotemporal expression of duplicate AGAMOUS orthologues during floral development in Phalaenopsis . Dev Genes Evol 216, 301–313 (2006). https://doi.org/10.1007/s00427-005-0057-0

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