Abstract
Main conclusion
Genetic transformation allows for greater bixin or norbixin production in achiote. Knowledge of genes that control the biosynthesis of these important secondary metabolites will allow for targeted amplification in transgenic plants.
Annatto is a natural dye or coloring agent derived from the seeds, or their arils, of achiote (Bixa orellana L.), and is commercially known as E160b. The main active component of annatto dye is water-insoluble bixin, although water-soluble norbixin also has commercial applications. Relative to other antioxidants, bixin is light- and temperature stable and is thus safe for human consumption. Bixin is, therefore, widely applied as a dye and as an antioxidant in the medico-pharmaceutical, food, cosmetic, and dye industries. Even though bixin has also been isolated from leaves and bark, yield is lower than from seeds. More biotechnology-based research of this industrial and medicinal plant is needed. Building on provisional genetic transformation studies, it would be advantageous to transform genes that could result in greater bixin or norbixin production. Reliable protocols for the extraction of bixin and norbixin, as well as deeper knowledge of the genes that control the biosynthesis of these important secondary metabolites will allow for targeted amplification in transgenic plants.
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs00425-018-2909-7/MediaObjects/425_2018_2909_Fig1_HTML.jpg)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs00425-018-2909-7/MediaObjects/425_2018_2909_Fig2_HTML.gif)
Similar content being viewed by others
References
Abayomi M, Adebayo AS, Bennett D, Porter R, Shelly-Campbell J (2014a) In vitro antioxidant activity of Bixa orellana (annatto) seed extract. J Appl Pharm Sci 4:101–106. https://doi.org/10.7324/JAPS.2014.40216
Abayomi M, Adebayo AS, Bennett D, Porter R, Shelly-Campbell J, Dawkin G (2014b) Phytochemical testing and in vitro antibacterial activity of Bixa orellana (annatto) seed extract. Br J Pharm Res 4:1387–1399. https://doi.org/10.9734/BJPR/2014/10925
Abdo BM (2015) Isolation, characterization and biological activities of food colorants from Bixa orellana. MSc thesis, Haramaya University, Ethiopia, pp 66
Alim S, Bairagi N, Shahriyar S, Kabir MM, Rahman MH (2016) In vitro antibacterial potential of Bixa orellana L. against some pathogenic bacteria and comparative investigation on some standard antibiotics. J Pharmacog Phytochem 5:178–181
Amaral LIV, de Pereira FA, Cortelazzo AL (1995) Dormancy breaking in seeds of Bixa orellana. Rev Bras Fisiol Vegetal 7:151–157 (in Portuguese with English abstract)
Antunes LMG, Pascoal LM, Bianchi MLP, Dias FL (2005) Evaluation of the clastogenicity and anticlastogenicity of the carotenoid bixin in human lymphocyte cultures. Mutat Res/Genet Toxicol Env Mutagen 585:113–119. https://doi.org/10.1016/j.mrgentox.2005.04.006
Aseervatham SB, Sasikumar JM, Kumar D (2012) Studies on in vitro free radical scavenging activity of Bixa orellana L. bark extract. Int J Pharmacy Pharmaceut Sci 4:719–726
Bouvier F, Dogbo O, Camara B (2003) Biosynthesis of the food and cosmetic plant pigment bixin (annatto). Science 300:2089–2091. https://doi.org/10.1126/science.1085162
Cárdenas-Conejo Y, Carballo-Uicab V, Lieberman M, Aguilar-Espinosa M, Comai L, Rivera-Madrid R (2015) De novo transcriptome sequencing in Bixa orellana to identify genes involved in methylerythritol phosphate, carotenoid and bixin biosynthesis. BMC Genom 16:877. https://doi.org/10.1186/s12864-015-2065-4
Carvalheira GMG, Guerra M, dos Santos GA, de Andrade VC, de Farias MCA (1991) Citogenética de angiospermas coletadas em Pernambuco-IV. Acta Bot Braz 5(2):37–51. https://doi.org/10.1590/S0102-33061991000200003
Chisté RC, Yamashita F, Gozzo FC, Mercadante AZ (2011) Simultaneous extraction and analysis by high performance liquid chromatography coupled to diode array and mass spectrometric detectors of bixin and phenolic compounds from annatto seeds. J Chromatogr A 1218:57–63. https://doi.org/10.1016/j.chroma.2010.10.094
Ciro GL, Zapata JE, López J (2014) In vitro evaluation of Bixa orellana L. (annatto) seeds as potential natural food preservative. J Med Plant Res 8:772–779. https://doi.org/10.5897/JMPR2013.5341
de Almeida PM, Carvalho CR, Clarindo WR (2006) Classical and molecular cytogenetic tools to resolve the Bixa karyotypes. Cytologia 71:391–398. https://doi.org/10.1508/cytologia.71.391
de Carvalho JFRP, de Carvalho CRP, Otoni WC (2005) In vitro induction of polyploidy in annatto (Bixa orellana). Plant Cell Tiss Organ Cult 80:69–75. https://doi.org/10.1007/s11240-004-8833-5
Dequigiovanni G, Ramos SLF, Zucchi MI, Bajay MM, Pinheiro JB, Fabri EG, Bressan EA, Veasey EA (2014) Isolation and characterization of microsatellite loci for Bixa orellana, an important source of natural dyes. Genetics Mol Res 13:9097–9102. https://doi.org/10.4238/2014.October.31.25
Dequigiovanni G, Ramos SLF, Alves-Pereira A, Fabri EG, Carvalho PRN, da Silva MG, Abdo MTVN, Martins ALM, Clement CR, Veasey EA (2017) Genetic diversity and structure in a major Brazilian annatto (Bixa orellana) germplasm bank revealed by microsatellites and phytochemical compounds. Genetic Res Crop Evol 64:1775–1788. https://doi.org/10.1007/s10722-017-0535-z
Di Mascio P, Devasagayam TP, Kaiser S, Sies HS (1990) Carotenoids, tocopherols and thiols as biological singlet molecular oxygen quenchers. Biochem Soc Trans 18:1054–1056. https://doi.org/10.1042/bst0181054
Dias NO, Rebouças TNH, São José AR, Amaral CLF (2017) Morpho-agronomic characterization and estimates of genetic parameters in annatto plant. Hortic Bras 35:242–246. https://doi.org/10.1590/S0102-053620170214
dos Santos GC, Mendonça LM, Antonucci GA, dos Santos AC, Antunes LMG, Bianchi MLP (2012) Protective effect of bixin on cisplatin-induced genotoxicity in PC12 cells. Food Chem Toxicol 50:335–340. https://doi.org/10.1016/j.fct.2011.10.033
Echevarría-Machado I, Sánchez-Cach LA, Hernández-Zepeda C, Rivera-Madrid R, Moreno-Valenzuela OA (2005) A simple and efficient method for isolation of DNA in high mucilaginous plant tissues. Mol Biotechnol 31:129–136. https://doi.org/10.1385/MB:31:2:129
Fernandes ACS, Almeida CA, Albano F, Laranja GAT, Felzenszwalb I, Lage CLS, de Sá CCNF, Moura AS, Kovary K (2002) Norbixin ingestion did not induce any detectable and breakage in liver and kidney but caused a considerable impairment in plasma glucose levels of rat and mice. J Nutr Biochem 13:411–420. https://doi.org/10.1016/S0955-2863(02)00177-8
Floss DS, Hause B, Lange PR, Ku H, Strack D, Walter MH (2008) Knock-down of the MEP pathway isogene 1-deoxy-d-xylulose 5-phosphate synthase 2 inhibits formation of arbuscular mycorrhiza-induced apocarotenoids, and abolishes normal expression of mycorrhiza-specific plant marker genes. Plant J 56:86–100. https://doi.org/10.1111/j.1365-313X.2008.03575x
Giridhar P, Parimalan R (2010) A biotechnological perspective towards improvement of annatto color production for value addition—the influence of biotic elicitors. Asia-Pacific J Mol Biol Biotechnol 18:77–79
Giridhar P, Mahendranath G, Venugopalan A, Ravishankar GA (2012) Enhanced yield of food colourant annatto from seeds of Bixa orellana L.: the efficacy of polyamines floral spray. Proc Nat Acad Sci India Sect B: Biol Sci 82:553–556. https://doi.org/10.1007/s40011-012-0058-5
Giridhar P, Venugopalan A, Parimalan R (2014) A review on annatto dye extraction, analysis and processing—a food technology perspective. J Sci Res Rep 3:327–348. https://doi.org/10.9734/JSRR/2014/5870
Guo PH, Zhao WE, Zhang X, Li QQ, Li X (2009) Effection [sic] of bixin on cell apoptosis of the K562 of preleukemia and expression of PPARγ protein. Chin Trad Herb Drugs 40:782–784 (in Chinese)
Gupta P (2016) Bixa orenalla [sic]: a review on its phytochemistry, traditional and pharmacological uses. World J Pharm Sci 4:500–510
Haila KM, Lievonen SM, Heinonen MI (1996) Effects of lutein, lycopene, annatto, and g-tocopherol on autoxidation of triglycerides. J Agric Food Chem 44:2096–2100. https://doi.org/10.1021/jf9504935
Hallagan JB, Allen DC, Borzelleca JF (1995) The safety and regulatory status of food, drug and cosmetics color additives exempt from certification. Food Chem Toxicol 33:515–528. https://doi.org/10.1016/0278-6915(95)00010-Y
Hanson L, McMahon KA, Johnson MAT, Bennet MD (2001) First nuclear DNA C-values for another 25 angiosperm families. Ann Bot 88:851–858. https://doi.org/10.1006/anbo.2001.1528
He MY, Li GH, Li HQ, Chen JB (2009) Study on preparation technology of bixin from annatto seeds in **shuangbanna. J Yunnan Agric Univ 24:243–249 (in Chinese with English abstract)
Jako C, Coutu C, Ingerborg R, Reed DW, Pelcher LE, Covello PS (2002) Probing carotenoid synthesis in develo** seed coats of Bixa orellana (Bixaceae) through expressed sequence tag analysis. Plant Sci 163:141–145. https://doi.org/10.1016/S0168-9452(02)00083-3
Júnior ACTS, Asad LMBO, de Oliveira EB, Kovary K, Nasser R, Asad NR, Felzenszwalb I (2005) Antigenotoxic and antimutagenic potential of an annatto pigment (norbixin) against oxidative stress. Gen Mol Res 4:94–99
Krishnan N, Ayyangar KR (1987) Taxonomical relationships of Bixa L. In: Proceedings of the Indian Science Congress Association Vol. 74(3, VI) p. 105
Lauro GJ, Francis FJ (eds) (2000) Natural food colorants. Marcel Dekker Inc., New York
Leal F, Michelangeli de clavijo CM (2010) Annatto: a natural dye from the tropics. Chron Hortic 50:34–36
Leal F, Michelangeli De Clavijo CM (2012) Annatto: botany and horticulture. In: Janick J (ed) Horticultural reviews, vol. 39, 1st edn. Wiley-Blackwell, New York, pp 389–419. https://doi.org/10.1002/9781118100592.ch8
Lobato KBS, Paese K, Forgearini JC, Guterres SS, Jablonski A, Rios AO (2013) Characterisation and stability evaluation of bixin nanocapsules. Food Chem 141:3906–3912. https://doi.org/10.1016/j.foodchem.2013.04.135
Lombello RA, Pinto-Maglio CAF (2014) Cytogenetics and reproductive biology of Bixa orellana L. (Bixaceae). Cytologia 79:379–386. https://doi.org/10.1508/cytologia.79.379
März U (2015) The global market for carotenoids. BCC Research Global Information Inc., Kawasaki, p 173
Mazzani E, Marín RC, Segovia V (2000) Estudio de la variabilidad existente en la colección de onoto (Bixa orellana L.) del CENIAP, FONAIAP, Venezuela. Rev Fac Agron (LUZ) 17:492–504 (in Spanish with English abstract)
Medina AM, Michelangeli C, Ramis C, Díaz A (2001a) Caracterización morfológica de frutos de onoto (Bixa orellana L.) y su correspondencia con patrones de proteínas e isoenzimas. Acta Cient Venez 52:14–23
Medina AM, Michelangeli C, Ramis C, Díaz A (2001b) Identificación y variabilidad genética de genotipos de onoto (Bixa orellana L.) mediante el uso de proteínas hidrosolubles e isoenzimas. Acta Cient Venez 52:24–33
Medina-Flores D, Ulloa-Urizar G, Camere-Colarossi R, Caballero-García S, Mayta-Tovalino F, del Valle-Mendoza J (2016) Antibacterial activity of Bixa orellana L. (achiote) against Streptococcus mutans and Streptococcus sanguinis. Asian Pac J Trop Biomed 6:400–403. https://doi.org/10.1016/j.apjtb.2016.03.005
Mercadante AZ, Steck A, Pfander H (1997) Isolation and identification of new apocarotenoids from annatto (Bixa orellana) seeds. J Agric Food Chem 45:1050–1054. https://doi.org/10.1021/jf960412k
Michelangeli C, Medina AM, Artioli P, Mata J (2002) Microsporogénesis y micogametogénesis de onoto (Bixa orellana L.). Acta Cient Venez 53:171–175
Montenegro MA, Rios AO, Mercadante AZ, Nazareno MA, Borsarelli CD (2004) Model studies on the photosensitized isomerization of bixin. J Agric Food Chem 52:367–373. https://doi.org/10.1021/jf0349026
Nag S, Mandal S (2014) A review work on origin and history, botanical description and agro-technology of the medicinal plant sinduri (Bixa orellana L.). Int Res J Nat Appl Sci 1:27–32
Narváez J, Flores P, Herrera-Valencia V, Castillo F, Ku-Cahuich R, Canto-Canché B, Santana-Buzzy N, Rivera-Madrid R (2001a) Development of molecular techniques for the study the metabolism of carotenoids in the high pigment producer plant Bixa orellana L. HortScience 36:982–986
Narváez JA, Canto-Canché B, Flores P, Rivera-Madrid R (2001b) Differential expression of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) during flower and fruit development of Bixa orellana. J Plant Physiol 158:1471–1477. https://doi.org/10.1078/0176-1617-00614
Natividad LR, Rafael RR (2014) Carotenoid analyses and antibacterial assay of annatto (Bixa oerellana L.), carrot (Daucus carota L.), corn (Zea mays L.) and tomato (Solanum lycopersicum L.) extracts. Res J Recent Sci 3(3):40–45
Ohri D, Bhargava A, Catterjee A (2004) Nuclear DNA amounts in 112 species of tropical hardwoods—new estimates. Plant Biol 6:555–561. https://doi.org/10.1055/s-2004-821235
Paiva Neto VB, Carvalho CR, Otoni WC (2003) Mannose: a potential selection system for genetic transformation of annatto. Biol Plant 47:441–444. https://doi.org/10.1023/B:BIOP.0000023891.16785.fc
Panchal CB, Sapkal EA, Choudhary HD, Padhiar JS, Deshmukh SN (2014) Determination of sun protecting factor of pigment isolated from Bixa orellana. Int J Pharma Res Scholars 3:228–231
Parimalan R, Venugopal A, Giridhar P, Ravishankar GA (2011) Somatic embryogenesis and Agrobacterium-mediated transformation in Bixa orellana L. Plant Cell Tiss Organ Cult 105:317–328. https://doi.org/10.1007/s11240-010-9870-x
Paumgartten FJR, de Carvalho RR, Araújo IB, Pinto FM, Borges OO, Souza CAM, Kuriyama SN (2002) Evaluation of the developmental toxicity of annatto in the rat. Food Chem Toxicol 40:1595–1601. https://doi.org/10.1016/S0278-6915(02)00133-3
Pech-Hoil R, Ferrer MM, Aguilar-Espinosa M, Valdez-Ojeda R, Garza-Caligaris LE, Rivera-Madrid R (2017) Variation in the mating system of Bixa orellana L. (achiote) under three different agronomic systems. Sci Hortic 223:31–37. https://doi.org/10.1016/j.scienta.2017.05.031
Peng G, Wang CY, Song S, Fu XM, Azam M, Grierson D, Xu CG (2013) The role of 1-deoxy-d-xylulose-5-phosphate synthase and phytoene synthase gene family in citrus carotenoid accumulation. Plant Physiol Biochem 71:67–76. https://doi.org/10.1016/j.plaphy.2013.06.031
Portela de Carvalho JFR, Robinson IP, Alfenas AC (2005) Isozymic variability in a Brazilian collection of annatto (Bixa orellana L.). Pesqui Agropec Bras 40:653–660. https://doi.org/10.1590/S0100-204X2005000700005
Preston HD, Rickard MD (1980) Extraction and chemistry of annatto. Food Chem 5:47–56. https://doi.org/10.1016/0308-8146(80)90063-1
Raddatz-Mota D, Pérez-Flores LJ, Carrari F, Mendoza-Espinoza JA, Díaz de León-Sánchez F, Pinzón-López LL, Godoy-Hernández G, Rivera-Cabrera F (2017) Achiote (Bixa orellana L.): a natural source of pigment and vitamin E. J Food Sci Technol 54:1729–1741. https://doi.org/10.1007/s13197-017-2579-7
Reith JF, Giellen JW (1971) Properties of bixin and norbixin and the composition of annatto extracts. J Food Sci 36:861–864. https://doi.org/10.1111/j.1365-2621.1971.tb15545.x
Rios AO, Mercadante AZ, Borsarelli CD (2007) Triplet state energy of the carotenoid bixin determined by photoacoustic calorimetry. Dyes Pigment 74:561–565. https://doi.org/10.1016/j.dyepig.2006.03.018
Rivera-Madrid R, Escobedo-GM RM, Balam-Galera E, Vera-Ku M, Harries H (2006) Preliminary studies toward genetic improvement of annatto (Bixa orellana L.). Sci Hortic 109:165–172. https://doi.org/10.1016/j.scienta.2006.03.011
Rivera-Madrid R, Burnell J, Aguilar-Espinosa M, Rodríguez-Ávila NL, Lugo-Cervantes E, Sáenz-Carbonell LA (2013) Control of carotenoid gene expression in Bixa orellana L. leaves treated with norflurazon. Plant Mol Biol Rep 31:1422–1432. https://doi.org/10.1007/s11105-013-0604-1
Rivera-Madrid R, Aguilar-Espinosa M, Cárdenas-Conejo Y, Garza-Caligaris LE (2016a) Carotenoid derivates in achiote (Bixa orellana) seeds: synthesis and health promoting properties. Front Plant Sci 7:1406. https://doi.org/10.3389/fpls.2016.01406
Rivera-Madrid R, Godoy-Hernández G, Aguilar-Espinosa M, Cárdenas-Conejo Y, Garza-Caligaris LE, Lugo-Cervantes E (2016b) Avances biotecnológicos para potenciar la producción de carotenoides de Bixa orellana (achiote) para su uso y aprovechamiento agroindustrial. Carotenoides en agroalimentación y salud. Meléndez Martínez AJ (ed) Terracota, México, D.F., México. ISBN: 978-84-15413-35-6 (in press)
Rocha Garcia CE, Bolognesi VJ, Gaspari Dias JF, Gomes MO, Klocker Costa C (2012) Carotenoides bixina e norbixina extraídos do urucum (Bixa orellana L.) como antioxidantes em produtos cárneos. Ciência Rural 42:1510–1517. https://doi.org/10.1590/S0103-84782012000800029 (in Portuguese with English abstract)
Rodrigues SM, Soares VLF, de Oliveira TM, Gesteira AS, Otoni WC, Costa MGC (2007) Isolation and purification of RNA from tissues rich in polyphenols, polysaccharides, and pigments of annatto (Bixa orellana L.). Mol Biotechnol 37:220–224. https://doi.org/10.1007/s12033-007-0070-9
Rodriguez-Ávila NL, Narvaez-Zapata JA, Aguilar-Espinosa ML, Rivera-Madrid R (2011) Regulation of pigment-related genes during flower and fruit development of Bixa orellana. Plant Mol Biol Rep 29:43–50. https://doi.org/10.1007/s11105-010-0207-z
Rodríguez-Ávila NL, Narváez-Zapata JA, Aguilar-Espinosa ML, Rivera-Madrid R (2009) Full-length gene enrichment by using an optimized RNA isolation protocol in Bixa orellana recalcitrant tissues. Mol Biotechnol 42:84–90. https://doi.org/10.1007/s12033-008-9138-4
Rodríguez-Ávila NL, Narváez-Zapata JA, Ramírez-Benítez JE, Aguilar-Espinosa ML, Rivera-Madrid R (2011) Identification and expression pattern of a new carotenoid cleavage dioxygenase gene member from Bixa orellana. J Exp Bot 62:5385–5395. https://doi.org/10.1093/jxb/err201
Saha PD, Sinha K (2012) Natural dye from bixa seeds as a potential alternative to synthetic dyes for use in textile industry. Desal Water Treat 40(1–3):298–301. https://doi.org/10.1080/19443994.2012.671169
Saladié M, Wright LP, Garcia-Mas J, Rodríguez-Concepción M, Phillips MA (2014) The 2-C-methylerythritol 4-phosphate pathway in melon is regulated byspecialized isoforms for the first and last steps. J Exp Bot 65:5077–5092. https://doi.org/10.1093/jxb/eru275
Sankari M, Hemachandran H, Anantharaman A, Babu S, Rivera-Madrid R, Doss GPC, Fulzele DP, Siva R (2016) Identifying a carotenoid cleavage dioxygenase 4a gene and its efficient Agrobacterium-mediated genetic transformation in Bixa orellana L. Appl Biochem Biotechnol 179:697–714. https://doi.org/10.1007/s12010-016-2025-8
Scotter MJ (2009) The chemistry and analysis of annatto food colouring: a review. Food Add Contam Part A 26:1123–1145. https://doi.org/10.1080/02652030902942873
Scotter MJ, Wilson LA, Appleton GP, Castle L (1998) Analysis of annato (Bixa orellana) food coloring formulations. 1. Determination products by high-performance liquid chromatography with photodiode array detection. J Agric Food Chem 46:1031–1038. https://doi.org/10.1021/jf970063+
Shahid-ul-Islam Rather LJ, Mohammad F (2016) Phytochemistry, biological activities and potential of annatto in natural colorant production for industrial applications—a review. J Adv Res 7:499–514. https://doi.org/10.1016/j.jare.2015.11.002
Silva CR, Antunes LMG, Bianchi MLP (2001) Antioxidant action of bixin against cisplatin-induced chromosome aberrations and lipid peroxidation in rats. Pharmacol Res 43:561–566. https://doi.org/10.1006/phrs.2001.0822
Simmonds N (1954) Chromosome behaviour in some tropical plants. Heredity 8:139–146. https://doi.org/10.1038/hdy.1954.11
Soares VLF, Rodrigues SM, de Oliveira TM, de Queiroz TO, Lima LS, Hora-Júnior BT, Gramacho KP, Micheli F, Cascardo JCM, Otoni WC, Gesteira AS, Costa MGC (2011) Unraveling new genes associated with seed development and metabolism in Bixa orellana L. by expressed sequence tag (EST) analysis. Mol Biol Rep 38:1329–1340. https://doi.org/10.1007/s11033-010-0234-8
Stoykova P, Stoeva-Popova P (2011) PMI (manA) as a nonantibiotic selectable marker gene in plant biotechnology. Plant Cell Tiss Organ Cult 105:141–148. https://doi.org/10.1007/s11240-010-9858-6
Sumathi P, Parvathi A (2011) Antibacterial potential of the aqueous and organic extracts of Bixa orellana L. Int J Pharma Bio Sci 2(2):B193–B202
Tamil Salvi A, Dinesh MG, Satyan RS, Chandrasekaran B, Rose C (2011) Leaf and seed extracts of Bixa orellana L. exert antimicrobial activity against bacterial pathogens. J Appl Pharm Sci 1:116–120
Thresiamma KC, George J, Kuttan R (1996) Protective effect of curcumin, ellagic acid and bixin on radiation induced genotoxicity. Indian J Exp Biol 34:845–847
Tirimanna ASL (1981) Study of the carotenoid pigments of Bixa orellana L. seeds by thin layer chromatography. Microchim Acta 76:11–16. https://doi.org/10.1007/BF01197299
Trujillo-Hdez JA, Cárdenas-Conejo Y, Aguilar-Espinosa M, Escobar Turriza P, Comai L, Rivera-Madrid R (2016) Functional polymorphism in lycopene beta-cyclase gene as molecular marker to predict bixin production in annatto. Mol Breed 36:135. https://doi.org/10.1007/s11032-016-0555-y
Ulbricht C, Windsor RC, Brigham A, Bryan JK, Conquer J, Costa D, Giese N, Guilford J, Higdon ERB, Holmes K, Isaac R, **gst S, Kats J, Peery L, Rusie E, Savinainen A, Schoen T, Stock T, Tanguay-Colucci S, Weissner W (2012) An evidence-based systematic review of annatto (Bixa orellana L.) by the natural standard research collaboration. J Diet Suppl 9:57–77. https://doi.org/10.3109/19390211.2012.653530
Valdez-Ojeda R, Hernandez-Stefanoni JL, Aguilar-Espinosa M, Rivera-Madrid R, Ortiz R, Quiros CF (2008) Assessing morphological and genetic variation in annatto (Bixa orellana L.) by sequence-related amplified polymorphism and cluster analysis. HortScience 43:2013–2017
Valdez-Ojeda R, Quiros CF, Aguilar-Espinosa ML, Rivera-Madrid R (2010) Outcrossing rates in annatto determined by sequence-related amplified polymorphism. Agron J 102:1340–1345. https://doi.org/10.2134/agronj2009.0510
Vedavathy S (2003) Annatto and its potential in international market. Nat Product Radiance 2(2):72–75
Venugopalan A, Giridhar P (2012) Bacterial growth inhibition potential of annatto plant parts. Asian Pacific J Trop Biomed 2(3):S1879–S1882. https://doi.org/10.1016/S2221-1691(12)60513-9
Venugopalan A, Giridhar P (2014) Mycoflora associated with seeds of Bixa orellana L. Afr J Microbiol Res 6:2091–2094. https://doi.org/10.5897/AJMR11.1358
Venugopalan A, Giridhar P, Ravishankar GA (2011a) Food, ethanobotanical [sic] and diversified applications of Bixa orellana L.: a scope for its improvement through biotechnological mediation. Indian J Fund Appl. Life Sci 1(4):9–31
Venugopalan A, Giridhar P, Ravishankar GA (2011b) Morphological diversity in Bixa orellana L. and variations in annatto pigment yield. J Hortic Sci Biotechnol 86:319–324. https://doi.org/10.1080/14620316.2011.11512767
Vilar DA, Vilar MSA, de Lima e Moura TFA, Raffin FN, de Oliveira MR, Franco CFO, de Athayde-Filho PF, Diniz MFFM, Barbosa-Filho JM (2014) Traditional uses, chemical constituents, and biological activities of Bixa orellana L.: a review. Sci World J 2014:857292. https://doi.org/10.1155/2014/857292
Zaldívar-Cruz JM (2004) Transformación genética del achiote (Bixa orellana L.) con el gen reportero uidA vía Agrobacterium tumefaciens. PhD thesis, Centro de Investigación Científica de Yucatán, A.C., Mexico, pp 104 pp
Zaldívar-Cruz JM, Ballina-Gómez H, Guerrero-Rodríguez C, Avilés-Berzunza E, Godoy-Hernández GC (2003) Agrobacterium-mediated transient transformation of annatto (Bixa orellana) hypocotyls with the GUS reporter gene. Plant Cell Tiss Organ Cult 73:281–284. https://doi.org/10.1023/A:1023037108705
Zarza-García A, Sauri-Duch E, Raddatz-Mota D, Cuevas-Glory LF, Pinzón-López LL, Rivera-Cabrera F, Mendoza-Espinoza JA (2017) Pharmacological, phytochemical and morphological study of three Mayan accessuons of Bixa orellana L. leaves. Emir J Food Agric 29:163–169. https://doi.org/10.9755/ejfa.2016-09-128
Zhai B, Clark J, Ling T, Connelly M, Medina-Bolivar F, Rivas F (2014) Antimalarial evaluation of the chemical constituents of hairy root cultures of Bixa orellana. Molecules 19:756–766. https://doi.org/10.3390/molecules19010756
Acknowledgements
We thank Margarita Aguilar-Espinosa, who provided some of the pictures. RRM thanks the Consejo Nacional de Ciencia y Tecnología (CONACYT) with grant no. 46541, 98508, 220259. to support some previously published findings that were described in this review.
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Conflicts of interest
The authors declare no conflicts of interest.
Rights and permissions
About this article
Cite this article
Teixeira da Silva, J.A., Dobránszki, J. & Rivera-Madrid, R. The biotechnology (genetic transformation and molecular biology) of Bixa orellana L. (achiote). Planta 248, 267–277 (2018). https://doi.org/10.1007/s00425-018-2909-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00425-018-2909-7