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Molecular characterization of pepper germin-like protein as the novel PR-16 family of pathogenesis-related proteins isolated during the resistance response to viral and bacterial infection

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Abstract

To understand the molecular defense mechanism controlling the hypersensitive response (HR) better, we examined the hot pepper plant (Capsicum annuum L. cv. Bugang), which exhibits an HR in response to infection by Tobacco mosaic virus pathotype P0 (TMV-P0). A full-length cDNA clone was isolated by differential screening of a cDNA library that was constructed with mRNA extracted from hot pepper leaves during the resistance response to TMV-P0. The predicted amino acid sequence of the cDNA clone exhibited a high sequence similarity to germin-like protein (GLP). The CaGLP1 (Capsicum annuum GLP1) cDNA contains a single open reading frame of 660 bp encoding 220 amino acid residues. Upon inoculation with TMV or Xanthomonas, CaGLP1 transcripts were specifically accumulated in the incompatible interaction but not in the compatible interaction. In plants treated with salicylic acid (SA) or ethephon, which are signal molecules in the defense-related signal transduction pathway, CaGLP1 transcripts were accumulated rapidly. As far as we know, this is the first report that plant GLPs can be specifically induced during a defense response against viral infection. These data suggest that in the hot pepper plant CaGLP1 may be involved in the defense response to viral pathogens, and thus be classified as a new family of PR proteins, ‘PR-16’.

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Abbreviations

CaGLP1:

Capsicum annuum germin-like protein

HR:

Hypersensitive response

MeJA:

Methyl jasmonate

PR:

Pathogenesis related

SA:

Salicylic acid

TMV :

Tobacco mosaic virus

Xcv :

Xanthomonas campestris pv. vesicatoria

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Acknowledgments

We thank the members of Dr. Paek’s laboratory for helpful discussions during the work. This study was supported partly by a grant (CG1223) from the Korea Ministry of Science and Technology, a grant from the Korea Science and Engineering Foundation to the Plant Signaling Network Research Center, and a Korea University Grant, Republic of Korea. C.-J. Park and J.-M. An contributed equally to this work.

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Authors and Affiliations

  1. School of Life Sciences and Biotechnology, Korea University, 1, 5-ga, Anam-dong, Sungbuk-gu, 136-701, Seoul, Republic of Korea

    Chang-** Park, Jong-Min An, Yun-Chul Shin, Ki-Jeong Kim, Boo-Ja Lee & Kyung-Hee Paek

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  1. Chang-** Park
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  2. Jong-Min An
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  3. Yun-Chul Shin
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  4. Ki-Jeong Kim
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  6. Kyung-Hee Paek
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Corresponding author

Correspondence to Kyung-Hee Paek.

Additional information

The nucleotide sequence data reported in this paper were submitted to the EMBL, GenBank and DDBJ Nucleotide Sequence Databases under the accession number AY391748

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Park, CJ., An, JM., Shin, YC. et al. Molecular characterization of pepper germin-like protein as the novel PR-16 family of pathogenesis-related proteins isolated during the resistance response to viral and bacterial infection. Planta 219, 797–806 (2004). https://doi.org/10.1007/s00425-004-1290-x

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  • DOI: https://doi.org/10.1007/s00425-004-1290-x

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