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Rab3A, Rab27A, and Rab35 regulate different events during mouse oocyte meiotic maturation and activation

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Abstract

Rab family members play important roles in membrane trafficking, cell growth, and differentiation. Almost all components of the cell endomembrane system, the nucleus, and the plasma membrane are closely related to RAB proteins. In this study, we investigated the distribution and functions of three members of the Rab family, Rab3A, Rab27A, and Rab35, in mouse oocyte meiotic maturation and activation. The three Rab family members showed different localization patterns in oocytes. Microinjection of siRNA, antibody injection, or inhibitor treatment showed that (1) Rab3A regulates peripheral spindle and cortical granule (CG) migration, polarity establishment, and asymmetric division; (2) Rab27A regulates CG exocytosis following MII-stage oocyte activation; and (3) Rab35 plays an important role in spindle organization and morphology maintenance, and thus meiotic nuclear maturation. These results show that Rab proteins play important roles in mouse oocyte meiotic maturation and activation and that different members exert different distinct functions.

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Acknowledgments

We appreciate Yi Hou, Shi-Wen Li, and Li-Juan Wang’s help for their technical assistance. We also thank the other members in Dr. Sun’s laboratory for their kind discussions of these experiments. This study was supported by the National Basic Research Program of China (No. 2012CB944404).

Authors’ contribution

QYS and HHW helped with conception and design. QC, TZ, ZBW, YCOY, WS, JYM, and HS contributed to acquisition of data. QYS and HHW contributed to analysis and interpretation of data, and drafting the article. All authors approved the final version of the manuscript.

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Authors and Affiliations

  1. College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China

    H. H. Wang & Q. Y. Sun

  2. State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Bei**g, 100101, China

    H. H. Wang, T. Zhang, Z. B. Wang, Y. C. Ouyang & Q. Y. Sun

  3. Institute of Reproductive Sciences, Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, 266109, China

    H. H. Wang, Q. Cui, T. Zhang, W. Shen, J. Y. Ma & Q. Y. Sun

  4. Department of Veterinary Pathobiology, University of Missouri, Columbia, MO, 65211, USA

    H. Schatten

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  1. H. H. Wang
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  2. Q. Cui
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Correspondence to Q. Y. Sun.

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Wang, H.H., Cui, Q., Zhang, T. et al. Rab3A, Rab27A, and Rab35 regulate different events during mouse oocyte meiotic maturation and activation. Histochem Cell Biol 145, 647–657 (2016). https://doi.org/10.1007/s00418-015-1404-5

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  • DOI: https://doi.org/10.1007/s00418-015-1404-5

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