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Weak link of tendon–bone healing and a control experiment to promote healing

  • Arthroscopy and Sports Medicine
  • Published:
Archives of Orthopaedic and Trauma Surgery Aims and scope Submit manuscript

Abstract

Objective

This study aims to observe the mechanical weak point and histological features of tendon–bone interface after anterior cruciate ligament reconstructive surgery and to explore the tendon–bone healing effects of the platelet-rich gel (PRG) + deproteinized bone (DPB) compound.

Methods

A total of 48 New Zealand white rabbits were randomly divided into normal group, model (without embedding), experimental (embedded with the PRG + DPB compound), and control (embedded with DPB) groups. The rabbits were executed at 2, 4, 8, and 12 weeks after the operation. Then, micro-computed tomography scan and uniaxial tensile test were conducted. The fractured specimens were subjected to histological observation.

Results

At 4, 8, and 12 weeks after the operation, the bone density of the tendon–bone bound section of the experimental group was higher than that of the other groups (P < 0.05). At 4 and 8 weeks, the maximum tensile load of the experimental group was obviously higher than that of the control and model groups (P < 0.05). Histological observation indicated that the tendon–bone interface in the experimental group had more cartilage and bone tissue growing toward the internal tendon, but the fracture layer mainly occurred in the non-ankylosed part.

Conclusion

The mechanical weak point of the early tendon–bone interface was in the immature fibrous tissue. The PRG + DPB compound can effectively trigger tendon–bone healing by promoting the maturation and ossification of the tendon–bone tissue. This compound improved the tensile strength of the healing interface and reduced bone tunnel enlargement.

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Acknowledgments

We extremely appreciate the patient guidance and substantial support in the material preparation in the aspect of mechanics, microanatomy and imaging, respectively, from teacher Weidong Zhao in biomechanical experiment of Nan**g Medical University, teacher Yihe Guo in pathology department of the 175th Military Hospital of PLA, teacher Lin Ouyang in Southeast Imaging Center of Southeast Hospital Affiliated to **amen University. Thanks for the hard work of all the workers in Animal Experiment Center of Southeast Hospital Affiliated to **amen University.

Conflict of interest

None.

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Authors and Affiliations

  1. Department of Orthopaedics, The Southeast Hospital Affiliated to **amen University, No. 269 Huazhong Road, Zhangzhou, Fujian, 363000, China

    Wenliang Zhai, Zhenqi Ding & Zhiwen Chen

  2. Fujian University of Traditional Chinese Medicine, Fuzhou, 350108, China

    Chenwei Lv, Yanmei Zheng & Yuechuan Gao

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  1. Wenliang Zhai
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  2. Chenwei Lv
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  3. Yanmei Zheng
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Corresponding author

Correspondence to Wenliang Zhai.

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Zhai, W., Lv, C., Zheng, Y. et al. Weak link of tendon–bone healing and a control experiment to promote healing. Arch Orthop Trauma Surg 133, 1533–1541 (2013). https://doi.org/10.1007/s00402-013-1802-x

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  • DOI: https://doi.org/10.1007/s00402-013-1802-x

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