SpringerLink
Log in

Structural characterization and antioxidant activity of lignin from sugarcane bagasse

  • Original Contribution
  • Published:
Colloid and Polymer Science Aims and scope Submit manuscript

Abstract

The aim of the present study was to extract lignin from sugarcane bagasse followed by its structural characterization, oxidation, and evaluation of antioxidant activities. Lignin was extracted in a process involving different concentrations (1, 5, and 10 %) of NaOH solutions. The chemical composition of untreated and treated bagasse samples was analyzed which clearly showed that the delignification was found to be maximum with 10 % NaOH. The scanning electron microscopy (SEM) images of untreated and treated samples of bagasse were taken to study the physical changes occurred in the bagasse during different treatments. Lignin extracted was purified with acidified water and oxidized with 10 % H2O2. Both lignin and oxidized lignin were structurally characterized using Fourier-transformed infrared (FTIR), 1H, and 13C NMR spectra. The antioxidant potential of lignin and its oxidized derivative was evaluated by radical scavenging activity using DPPH method. Lignin was found to have higher antioxidant activity than oxidized lignin. This was attributed to a higher number of phenolic groups in lignin than in oxidized lignin. Both lignin and its oxidized derivative were observed to have higher antioxidant activity than 3,5-di-tert-butyl-4-hydroxytoluene (BHT) and lower than 3-tert-butyl-4-hydroxyanisole (BHA). It was concluded that sugarcane bagasse lignin and its oxidized derivative could be used as potential antioxidant of food oils and fats.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Sun RC, Fang JM, Tomkinson J (2000) Structural and physico-chemical characterization of lignins solubilized during alkaline peroxide treatment of barley straw. Ind Crop Prod 12:71–83

    Article  CAS  Google Scholar 

  2. Uppal SK, Kaur R, Sharma P (2011) Optimization of chemical pretreatment and acid saccharification for conversion of sugarcane bagasse to ethanol. Sugar Tech 13:214–219

    Article  CAS  Google Scholar 

  3. Jayapal N, Samanta AK, Kolte AP, Senani S, Sridhar M, Suresh KP, Sampath KT (2013) Value addition to sugarcane bagasse: xylan extraction and its process optimization for xylooligosaccharides production. Ind Crop Prod 42:14–24

    Article  CAS  Google Scholar 

  4. Uppal SK, Kaur R (2011) Hemicellulosic furfural production from sugarcane bagasse using different acids. Sugar Tech 13:166–169

    Article  CAS  Google Scholar 

  5. Argyropoulos DS, Menachem SB (1998) Lignin. In: Kaplan DL (ed). Biopolymers from renewable resources. Springer: Berlin, pp 292

  6. Buranov AU, Mazza G (2008) Lignin in straw of herbaceous crops. Ind Crop Prod 28:237–259

    Article  CAS  Google Scholar 

  7. Sun RC, Fang JM, Tomkinson J, Bolton J (1999) Physicochemical and structural characterization of alkali soluble lignins from oil palm trunk and empty fruit-bunch fibers. J Agric Food Chem 47:2930–2936

    Article  CAS  Google Scholar 

  8. Fredheim GE, Braaten SM, Bjorn E, Christensen (2002) Molecular weight determination of lignosulfonates by size-exclusion chromatographt and multi-angle laser light scattering. J Chromatogr A 942 :191-199

  9. Mansouri NEE, Salvadó J (2006) Structural characterization of technical lignins for the production of adhesives: application to lignosulfonate, kraft, soda anthraquinone, organosolv and ethanol process lignins. Ind Crop Prod 24:08–16

    Article  Google Scholar 

  10. Dizhbite T, Telysheva G, Jurkjane V, Viesturs U (2004) Characterization of the radical scavenging activity of lignins-natural antioxidants. Bioresour Technol 95:309–317

    Article  CAS  Google Scholar 

  11. Pan X, Kadla JF, Ehara K, Gilkes N, Saddler JN (2006) Organosolv ethanol lignin from hybrid poplar as a radical scavenger: relationship between lignin structure, extraction conditions and antioxidant activity. J Agric Food Chem 54:5806–5813

    Article  CAS  Google Scholar 

  12. Catignani GL, Carter ME (1982) Antioxidant properties of lignin. J Food Sci 47:1745–1748

    Article  CAS  Google Scholar 

  13. Bjørsvik HR, Liguori L (2002) Organic processes to pharmaceutical chemicals based on fine chemicals from lignosulfonates. Org Process Res Dev 6:279–290

    Article  Google Scholar 

  14. Dong X, Dong M, Lu Y, Turley A, ** T, Wu C (2011) Antimicrobial and antioxidant activities of lignin from residue of corn stover to ethanol production. Ind Crop Prod 34:1629–1634

    Article  CAS  Google Scholar 

  15. Khanam LAM, Talukder D, Hye MA (2006) Toxic and repellent action of sugarcane bagasse-based lignin against some stored grain insect pests. Univ J Zool Rajshahi Univ 25:27–30

    Google Scholar 

  16. Senba Y, Nishishita T, Saito K, Yoshioka H, His Y (1999) Stopped-flow and spectrophotometric study on radical scavenging by tea catechins and the model compounds. Chem Pharm Bull 47:1369–1374

    Article  CAS  Google Scholar 

  17. Goering HK, Van Soest PJ (1970) Forage fibre analysis. Agricultural Handbook No 379

  18. Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton J, Crocker D (2011) Determination of structural carbohydrates and lignin in biomass. Laboratory Analytical Procedure 4:01-15

  19. Saha BC (2003) Hemicellulose bioconversion. J Ind Microbiol Biotechnol 30:279–291

    Article  CAS  Google Scholar 

  20. Peng F, Ren JL, Xu F, Bian J, Peng P, Sun RC (2009) Comparative study of hemicelluloses obtained by graded ethanol precipitation from sugarcane bagasse. J Agric Food Chem 57:6305–6317

    Article  CAS  Google Scholar 

  21. Rezende CA, de Lima MA, Maziero P, deAzevedo ER, Garcia W, Polikarpov I (2011) Chemical and morphological characterization of sugarcane bagasse submitted to a delignification process for enhanced enzymatic digestibility. Biotechnol Biofuels 4:02–18

    Article  Google Scholar 

  22. Yuan TQ, Sun S, Xu F, Sun RC (2011) Isolation and physio-chemical characterization of lignin from ultrasound irradiated fast-growing poplar wood. Bioresources 6:414–433

    CAS  Google Scholar 

  23. Barclay LRC, ** F, Norris JQ (1997) Antioxidant properties of phenolic lignin model compounds. J Wood Chem Technol 17:73–90

    Article  CAS  Google Scholar 

  24. Sun SL, Wen JL, Ma MG, Sun RC, Jones GL (2014) Structural features and antioxidant activities of degraded lignins fromsteam exploded bamboo stem. Ind Crop Prod 56:128–136

    Article  CAS  Google Scholar 

  25. Faustino H, Gil N, Baptista C, Duarte AP (2010) Antioxidant activity of lignin phenolic compounds extracted from kraft and sulphite black liquors. Molecules 15:9308–9322

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry, Punjab Agricultural University, Ludhiana, 141004, India

    Ramandeep Kaur & S. K. Uppal

Authors
  1. Ramandeep Kaur
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. K. Uppal
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ramandeep Kaur.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kaur, R., Uppal, S.K. Structural characterization and antioxidant activity of lignin from sugarcane bagasse. Colloid Polym Sci 293, 2585–2592 (2015). https://doi.org/10.1007/s00396-015-3653-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00396-015-3653-1

Keywords

Search

Navigation