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Olive oil improves the intestinal absorption and bioavailability of lutein in lutein-deficient mice

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Abstract

Purpose

To investigate the influence of olive (OO), groundnut (GNO), soybean (SBO), sunflower (SFO), rice bran (RBO), corn (CO), palm (PO) oil or mixed micelle (control) on absorption kinetics and bioavailability of lutein in lutein-deficient mice. Additional aim was to correlate the activity of intestinal triacylglycerol lipase with intestinal and plasma lutein levels.

Methods

After induction of lutein deficiency, mice (n = 165) were divided into eight groups (OO, SFO, GNO, RBO, PO, CO, SBO and control; n = 20/group) and the remaining (n = 5) were used as baseline (0 h). Groups were further divided into four subgroups (n = 5/subgroup) and were intubated with lutein (200 μM) dispersed in different vegetable oils. Plasma and tissue (intestine, liver and eyes), lutein, triglycerides, intestinal triacylglycerol lipases and fatty acid profile of plasma and tissues were measured at different time intervals.

Results

The percentage area under the curve value for plasma lutein in OO and GNO was higher by 41.8 and 5.1 %, while it was lower in other groups (18.2–53.3 %), when compared to control. Similarly, the percentage area under the curve for eye lutein in OO and GNO groups was higher by 35.2 and 4.8 %, whereas in other groups it was lower (5.4–69 %) than in control. Results show that olive oil facilitates the lutein absorption more compared to other vegetable oils, which may be due to the difference in fatty acid composition and higher activity of intestinal triacylglycerol lipase.

Conclusions

Dietary olive oil rich in oleic acid improves the bioavailability and accumulation of lutein in lutein-deficient mice by modifying the intestinal triacylglycerol lipase activity.

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Acknowledgments

The authors thank the Director and Head of the Department of Biochemistry and Nutrition, CSIR-CFTRI, for their encouragement. Nidhi Bhatiwada and Mamatha BS acknowledge the award of Senior Research Fellowship by University Grants Commission and Indian Council of Medical Research, Government of India, New Delhi, India. We would like to thank Dr. NS Mahendrakar, Chief Editor, Journal of Food Science and Technology (Springer), for critically reviewing the manuscript for English language.

Conflict of interest

The authors declare no conflict of interest to disclose that could influence this work.

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Authors and Affiliations

  1. Department of Biochemistry and Nutrition, CSIR-Central Food Technological Research Institute, Mysore, 570 020, Karnataka, India

    Bhatiwada Nidhi, Bangera Sheshappa Mamatha & V. Baskaran

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  1. Bhatiwada Nidhi
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  2. Bangera Sheshappa Mamatha
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  3. V. Baskaran
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Correspondence to V. Baskaran.

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Nidhi, B., Mamatha, B.S. & Baskaran, V. Olive oil improves the intestinal absorption and bioavailability of lutein in lutein-deficient mice. Eur J Nutr 53, 117–126 (2014). https://doi.org/10.1007/s00394-013-0507-9

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  • DOI: https://doi.org/10.1007/s00394-013-0507-9

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