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Peritoneal fluid biomarkers in the detection of colorectal anastomotic leaks: a systematic review

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Abstract

Purpose

Anastomotic leak (AL) in colorectal surgery leads to significant morbidity, mortality and poorer oncological outcomes. Diagnosis of AL is frequently delayed as current methods of detection are not 100% sensitive or specific. ‘Biomarkers’, such as cytokines and markers of ischaemia, from the milieu of the anastomosis may aid early detection. This paper aims to review the evidence for their role in AL detection, allowing identification of targets for future research.

Methods

A systematic review was performed using PubMed, MEDLINE and Cochrane Library databases. Papers concerning detection or prediction of AL with biomarkers were identified. References within the papers were used to identify further relevant articles.

Results

Research has taken place in small cohorts with varying definitions of AL. Lactate has consistently been shown to be elevated in patients with intra-abdominal complications and ALs. pH on post-operative day 3 showed excellent specificity. Despite mixed results, a meta-analysis found that the cytokines tumour necrosis factor-α and interleukin-6 were elevated early in AL. Detection of bacteria in drain fluid by RT-PCR has good specificity but a high rate of false positives.

Conclusions

Peritoneal cytokines, lactate and pH have the potential to identify AL early. The consistency of the results for lactate and pH, alongside the fact that they are easy, quick and inexpensive to test, makes them the most attractive targets. Studies in larger cohorts with standardized definitions of AL are required to clarify their usefulness. Emerging biosensor technology may facilitate the development of small, low-cost and degradable intra-abdominal devices to measure peritoneal fluid biomarkers.

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Acknowledgements

We gratefully acknowledge the financial assistance from grants from The Aileen Lynn Bequest Fund, Royal College of Physicians and Surgeons of Glasgow and from the Ileostomy Association, West of Scotland and UK.

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Authors and Affiliations

  1. Department of General Surgery, Royal Alexandra Hospital, Ward 26 Day Room, Corsebar Road, Paisley, PA2 9PN, UK

    Emma C. Wright, Mark Vella & Susan Moug

  2. Department of Biomedical Engineering, Wolfson Centre, University of Strathclyde, Glasgow, G4 0NW, UK

    Patricia Connolly

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  1. Emma C. Wright
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Correspondence to Emma C. Wright.

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Wright, E.C., Connolly, P., Vella, M. et al. Peritoneal fluid biomarkers in the detection of colorectal anastomotic leaks: a systematic review. Int J Colorectal Dis 32, 935–945 (2017). https://doi.org/10.1007/s00384-017-2799-3

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