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Pregnancy outcome in ethanol-treated mice with folic acid supplementation in saccharose

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Abstract

Introduction

Maternal folic acid deficiency is the most important metabolic factor in the etiology of neural tube defects (NTD) and is reduced by ethanol, which is extensively consumed by young women.

Objective

The objective of the study was to determine whether folic acid supplementation in dietary saccharose is efficient in the prevention NTD induced by ethanol in fetuses of Swiss mice.

Materials and methods

Pregnant mice were divided into four groups of six animals each: control (C), ethanol (E), deficient-supplemented (DS), and deficient-supplemented + ethanol (DSE). Groups C and E received commercial mouse chow (containing 3 mg/kg folic acid) throughout the experiment, while groups DS and DSE received a folic acid-free diet with the addition of saccharose supplemented with folic acid (2 mg/kg folic acid) in water. Group E and DSE animals received ethanol (4 g/kg) administered intraperitoneally from the seventh to the ninth gestational day (gd) and were euthanized on the 18th gd, while groups C and DS received saline.

Results

Congenital anomalies were observed in groups E and DSE. The fetal weight and length of the animals in group E were lower than in groups C and DS and, in group DSE, were lower than in groups C and DS. The placental diameter of group E was smaller than that of group C, and the placental weight of group C animals was lower than that of groups E, DSE, and DS.

Conclusion

The study demonstrated that dietary supplementation with folate in saccharose is an accessible means of consumption that could be further diffused but in an increased dose than recommended to reduce the teratogenic effects of ethanol.

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Acknowledgment

Research supported by a FUPEME grant to M. Y. Yanaguita and by FAEPA.

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Authors and Affiliations

  1. Department of Pathology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil

    M. Y. Yanaguita, C. M. Gutierrez, C. N. M. Ribeiro, G. A. Lima & L. C. Peres

  2. Department of Surgery (Division of Pediatric Neurosurgery), Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil

    H. R. Machado

  3. Division of Pediatric Neurosurgery, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, 14049-900, Ribeirão Preto, SP, Brazil

    H. R. Machado

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  1. M. Y. Yanaguita
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  2. C. M. Gutierrez
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  3. C. N. M. Ribeiro
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  4. G. A. Lima
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Correspondence to H. R. Machado.

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Yanaguita, M.Y., Gutierrez, C.M., Ribeiro, C.N.M. et al. Pregnancy outcome in ethanol-treated mice with folic acid supplementation in saccharose. Childs Nerv Syst 24, 99–104 (2008). https://doi.org/10.1007/s00381-007-0427-1

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  • DOI: https://doi.org/10.1007/s00381-007-0427-1

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