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LncRNAs PSMG3-AS1 and MEG3 negatively regulate each other to participate in endometrial carcinoma cell proliferation

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Abstract

Endometrial carcinoma (EC), also known as corpus cancer or corpus uterine cancer, is the most frequently diagnosed genital cancer among women in developed countries. Our preliminary RNA-seq analysis revealed the inverse correlation between the expression of PSMG3-AS1 and MEG3 across EC tissues, indicating the possible interaction between them. This study aimed to explore the interaction between two long non-coding RNAs (lncRNAs) PSMG3-AS1 and MEG3 in EC. Investigation of the interaction between two lncRNAs in cancer biology is a novel topic. The expression of PSMG3-AS1 and MEG3 in EC and paired non-tumor tissues from 60 EC patients were determined by RT-qPCR. Correlations between them were analyzed by Pearson’s correlation coefficient. PSMG3-AS1 and MEG3 were overexpressed in EC cells to study the relationship between them. The roles of PSMG3-AS1 and MEG3 in regulating the proliferation of EC cells were assessed by CCK-8 assay. PSMG3-AS1 was upregulated, while MEG3 was downregulated in EC. Across EC tissues, the expression of PSMG3-AS1 and MEG3 were inversely correlated. In EC cells, overexpression of PSMG3-AS1 and MEG3 resulted in the downregulation of each other. In cell proliferation assay, PSMG3-AS1 promoted cell proliferation, and MEG3 inhibited cell proliferation. Moreover, the proliferation rate of cells co-transfected with PSMG3-AS1 and MEG3 expression vectors was not different from that in cells without transfections. In conclusion, PSMG3-AS1 and MEG3 may negatively regulate each other to regulate EC cell proliferation.

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Data availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

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Funding

We thank the Fundamental Research Funds for the Central Universities (No. 3332019124).

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Authors and Affiliations

  1. Department of Gynecology and Obstetrics, Bei**g Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, No. 1 Dahua Road, Dongdan, Bei**g, 100730, People’s Republic of China

    Shuai Huang, Jiankun Chen, Xuexiao Gao, Zhiyuan Shang, **ao Ma, **a Zhang, Jiayang Li, Ruoyun Yin & **ao**g Meng

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  1. Shuai Huang
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  2. Jiankun Chen
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  4. Zhiyuan Shang
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  7. Jiayang Li
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  8. Ruoyun Yin
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  9. **ao**g Meng
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Contributions

SH designed the study. Shuai Huang carried out experiments and wrote the manuscript, SH revised the paper, and JC, XG, ZS, XM, XZ, JL, RY, and XM collected patient specimens and related information. JC, XG, ZS, XM, XZ, JL, RY, and XM contributed to analyzing the data. All authors reviewed the results and approved the final version of the manuscript.

Corresponding author

Correspondence to Shuai Huang.

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Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

The present study was approved by the Ethics Committee of Bei**g Hospital, National Center of Gerontology; Institute of Geriatric Medicine, Chinese Academy of Medical Sciences. The research has been carried out in accordance with the World Medical Association Declaration of Helsinki.

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All patients and healthy volunteers provided written informed consent prior to their inclusion within the study.

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Supplementary Information

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335_2021_9931_MOESM1_ESM.tif

Supplemental Figure 1 Expression of PSMG3-AS1 and MEG3 in normal EPC, RL95-2, and HEC-1-A cell lines. RT-qPCR was performed to analyze the expression of PSMG3-AS1(A) and MEG3 (B) in normal EPC, RL95-2, and HEC-1-A cell lines. Data of 3 biological replicates of multiple transfection groups were presented as mean ± SD values.*, p < 0.05. Supplementary file1 (TIF 362 KB)

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Huang, S., Chen, J., Gao, X. et al. LncRNAs PSMG3-AS1 and MEG3 negatively regulate each other to participate in endometrial carcinoma cell proliferation. Mamm Genome 33, 502–507 (2022). https://doi.org/10.1007/s00335-021-09931-y

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  • DOI: https://doi.org/10.1007/s00335-021-09931-y

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