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Overexpression of sugarcane gene SoSnRK2.1 confers drought tolerance in transgenic tobacco

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Abstract

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Overexpression of SoSnRK2.1 improved drought tolerance and growth of tobacco plants.

Abstract

Sucrose non-fermenting1-related protein kinase 2 (SnRK2) is a key enzyme in regulating ABA signal transduction in plants, and it plays a significant role in response to multiple abiotic stresses. In this research, SoSnRK2.1 gene was cloned from sugarcane variety GT21 and characterized under various stresses. The cloned SoSnRK2.1 gene has a complete open reading frame of 1002 bp, encoding a peptide of 333 amino acids. The amino acid sequence of SoSnRK2.1 has high homology with those of Zea mays and Oryza sativa, which belongs to SnRK2 s families. The expression of SoSnRK2.1 under stresses of drought, PEG, and ABA indicated that this gene is involved in stress responses in sugarcane. To investigate the gene function, fusional SoSnRK2.1-GFP-pBI121 under control of CaMV 35S was transformed into tobacco plants. Growth and morphology of transgenic plants demonstrated that overexpression of SoSnRK2.1 enhanced drought tolerance in tobacco. Transgenic tobacco plants had lower levels of ion leakage (IL), and contents of maleic dialdehyde (MDA) and H2O2, with higher activities of three antioxidant enzymes, superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), and chlorophyll and relative water content (RWC) than those in wide type (WT) tobacco. SoSnRK2.1 was stably transmitted to the next generation via sexual reproduction. Though the data presented here are from a heterologous system, it is highly likely that SoSnRK2.1 is involved in the abiotic stress response in sugarcane and may be playing an important role in regulation of its growth.

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Abbreviations

AA:

Amino acid

AMPK:

AMP-activated protein kinase

At:

Arabibopsis thaliana

AT:

Antisense transgenic

Bd:

Brachypodium distachyon

CAT:

Catalase

cDNA:

Complementary DNA

CDPK:

Calcium-dependent protein kinase

CTAB:

Cetyl trimethyl ammonium bromide

DIG:

Digoxigenin

DW:

Dry weight

FW:

Fresh weight

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

Gm:

Glycine max

IL:

Ion leakage

MDA:

Maleic dialdehyde

Nt:

Nicitiaba tabacum

Os:

Oryza sativa

pI:

Isoelectric point

POD:

Peroxidase

PP2C:

2C protein phosphatase

PYL:

Pyrabatin resistance 1-like

PYR:

Pyrabatin resistance

RACE:

Rapid amplification of cDNA ends

RCAR:

Regulatory component of ABA receptors

ROS:

Reactive oxygen species

RWC:

Relative water content

Sb:

Sorghum bicolor

Ser/Thr:

Serine/threonine protein kinase

SNF1:

Sucrose non-fermenting 1

SnRK2:

Sucrose non-fermenting1-related protein kinase 2

So:

Saccharum officinarum

SOD:

Superoxide dismutase

SOS2:

Salt over sensitive 2

ST:

Sense transgenic

TBA:

Thiobarbituric acid

TW:

Turgid weight

Vv:

Vitis vinifera

WT:

Wild type

Zm:

Zea mays

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Acknowledgments

The present study was supported by the grants from the National High Technology Research and Development Program (“863” Program) of China (2013AA102604), Natural Science Foundation of China (31360293), International Scientific Cooperation Program of China (2013DFA31600), Guangxi Special Funds for Bagui Scholars and Distinguished Experts (2013), and Guangxi Natural Science Fund (2011GXNSFF018002, 2012GXNSFDA053011).

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Authors and Affiliations

  1. Agricultural College, State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, 530005, China

    Thi-Thu Phan, Bo Sun, Jun-Qi Niu, Qin-Liang Tan, Jian Li, Li-Tao Yang & Yang-Rui Li

  2. Guangxi Key Laboratory of Sugarcane Genetic Improvement, Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Sugarcane Research Center, Chinese Academy of Agricultural Sciences, Guangxi Academy of Agricultural Sciences, Nanning, 530007, China

    Li-Tao Yang & Yang-Rui Li

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  1. Thi-Thu Phan
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The manuscript entitled ‘‘Overexpression of sugarcane gene SoSnRK2.1 confers drought tolerance in transgenic tobacco’’ has not been submitted to more than one journal for simultaneous consideration. Authors declare that this manuscript has not been published previously (partly or in full). The results of this study have not been split up into several parts. Authors also declare that data have not been fabricated or manipulated (including images) to support our conclusions. Data, text, or theories by others are not presented unless they are the author’s own. All authors whose names appear on the submission have contributed sufficiently to the scientific work and therefore share collective responsibility and accountability for the results.

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Phan, TT., Sun, B., Niu, JQ. et al. Overexpression of sugarcane gene SoSnRK2.1 confers drought tolerance in transgenic tobacco. Plant Cell Rep 35, 1891–1905 (2016). https://doi.org/10.1007/s00299-016-2004-0

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