Abstract
Key message
By comparing series full-length cDNA libraries stressed and control, the dynamic process of salt stress response in Upland cotton was studied, and reactive oxygen species and gibberellins signaling pathways were proposed.
Abstract
The Upland cotton is the most important fiber plant with highly salt tolerance. However, the molecular mechanism underlying salt tolerance in domesticated cotton was unclear. Here, seven full-length cDNA libraries were constructed for seedling roots of Upland cotton ‘Zhong G 5’ at 0, 3, 12 and 48 h after the treatment of control or 150 mM NaCl stress. About 3300 colonies in each library were selected robotically for 5′-end pyrosequencing, resulting in 20,358 expressed sequence tags (ESTs) totally. And 8516 uniESTs were then assembled, including 2914 contigs and 5602 singletons, and explored for Gene Ontology (GO) function. GO comparison between serial stress libraries and control reflected the growth regulation, stimulus response, signal transduction and biology regulation processes were conducted dynamically in response to salt stress. MYB, MYB-related, WRKY, bHLH, GRAS and ERF families of transcription factors were significantly enriched in the early response. 65 differentially expressed genes (DEGs), mainly associated with reactive oxygen species (ROS) scavenging, gibberellins (GAs) metabolism, signal transduction, transcription regulation, stress response and transmembrane transport, were identified and confirmed by quantitative real-time PCR. Overexpression of selected DEGs increased tolerance against salt stress in transgenic yeast. Results in this study supported that a ROS–GAs interacting signaling pathway of salt stress response was activated in Upland cotton. Our results provided valuable gene resources for further investigation of the molecular mechanism of salinity tolerance.
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Abbreviations
- ABA:
-
Abscisic acid
- BP:
-
Biological process
- BR:
-
Brassinosteroids
- CC:
-
Cellular components
- CDPK:
-
Calcium-dependent protein kinase
- CK:
-
Control library
- DEGs:
-
Differentially expressed genes
- Dof:
-
DNA binding with one finger
- ESTs:
-
Expressed sequence tags
- GAs:
-
Gibberellins
- GO:
-
Gene Ontology
- GRAS:
-
GAI [(Gibberellic acid)-insensitive], RGA (Repressor of GAI) and SCR (SCARECROW)
- MAPK:
-
Mitogen-activated protein kinase
- MF:
-
Molecular functions
- NR:
-
Stress library
- qRT-PCR:
-
Quantitative reverse transcriptase polymerase chain reaction
- ROS:
-
Reactive oxygen species
- TFs:
-
Transcription factors
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Acknowledgments
We thank Dr. Lida Zhang (Shanghai Jiaotong University), Dr. Yi Huang (Oil Crops Research Institute, Chinese Academy of Agricultural Sciences) and Dr. **nwang Wang (Texas A&M AgriLife Research and Extension Center, Texas A&M University System, Dallas, TX, 75252, USA) for helpful discussion. We are grateful to Dr. Kunbo Wang (Chinese Academy of Agricultural Sciences) for gifting us the seeds of Zhong G5. This research was supported in part by the National Key Project (2014ZX08005-004B), and National High Technology Research and Development Program (2006AA10A108) to J HUA.
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Shi, G., Guo, X., Guo, J. et al. Analyzing serial cDNA libraries revealed reactive oxygen species and gibberellins signaling pathways in the salt response of Upland cotton (Gossypium hirsutum L.). Plant Cell Rep 34, 1005–1023 (2015). https://doi.org/10.1007/s00299-015-1761-5
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DOI: https://doi.org/10.1007/s00299-015-1761-5