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Tissue culture-mediated biotechnological intervention in pomegranate: a review

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Abstract

The past 30 years have witnessed a series of systematic biotechnological advances made in pomegranate. These encompass optimization and establishment of in vitro culture techniques including micropropagation, somatic embryogenesis, synthetic seed production, plant regeneration via callus-mediated shoot organogenesis, adventitious shoot regeneration, anther culture, tetraploid induction and genetic transformation. This review attempts to provide a comprehensive account on the tissue culture-mediated biotechnological interventions made in pomegranate aimed at complementing conventional programmes for improvement of this nutraceutically important fruit crop.

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Abbreviations

AC:

Activated charcoal

AS:

Adenine sulfate

AVG:

Aminoethoxyvinylglycine

BAP:

6-Benzylaminopurine

B5 :

Gamborg’s B5 (1968) medium

CH:

Casein hydrolysate

CW:

Coconut water

2,4-D:

2,4-Dichlorophenoxyacetic acid

GA3 :

Gibberellic acid

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

IPA:

Indole-3-propionic acid

Kin:

Kinetin

MS:

Murashige and Skoog (1962) medium

NAA:

Naphthaleneacetic acid

PGRs:

Plant growth regulators

PVP:

Polyvinylpyrrolidone

WPM:

Lloyd and McCown (1980) woody plant medium

ZR:

Zeatin riboside

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Acknowledgments

The authors acknowledge the copyright permission from ‘Elsevier’ for reproducing some photographs of Fig. 2 as follows. Figure 2a: Reprinted from ‘Scientia Horticulturae’, vol. 79, Naik SK, Pattnaik S, Chand PK, In vitro propagation of pomegranate (Punica granatum L. cv. Ganesh) through axillary shoot proliferation from nodal segments of mature tree, pp. 175–183, Copyright (1999), with permission from Elsevier; Fig. 2b: reprinted from ‘Scientia Horticulturae’ vol. 85, Naik SK, Pattnaik S, Chand PK, High frequency axillary shoot proliferation and plant regeneration from cotyledonary nodes of pomegranate (Punica granatum L.), pp. 261–270, Copyright (2000), with permission from Elsevier; Fig. 2h, i and j: Reprinted from ‘Journal of Plant Physiology’, vol. 160, Naik SK, Chand PK, Silver nitrate and aminoethoxyvinylglycine promote in vitro adventitious shoot regeneration of pomegranate (Punica granatum L.), pp. 423–430, Copyright Elsevier (2003), with permission from Global Rights::Elsevier.

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Authors and Affiliations

  1. Department of Botany, Ravenshaw University, Cuttack, 753003, Orissa, India

    Soumendra K. Naik

  2. Plant Cell and Tissue Culture Facility, Post Graduate Department of Botany, Utkal University, Vani Vihar, Bhubaneswar, 751004, Orissa, India

    Pradeep K. Chand

Authors
  1. Soumendra K. Naik
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  2. Pradeep K. Chand
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Correspondence to Pradeep K. Chand.

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Communicated by R. Reski.

A contribution to the Special Issue: Plant Biotechnology in Support of the Millennium Development Goals.

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Naik, S.K., Chand, P.K. Tissue culture-mediated biotechnological intervention in pomegranate: a review. Plant Cell Rep 30, 707–721 (2011). https://doi.org/10.1007/s00299-010-0969-7

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