Abstract
Most Fusarium head blight (FHB) resistant barley (Hordeum vulgare L.) accessions perform relatively poorly from an agronomic point of view. Due to the polygenic inheritance of FHB resistance, introgression of this complex trait into well-adapted elite germplasm will likely require multiple cycles of hybridization and selection to combine resistance and agronomic performance. The use of anther culture to produce doubled haploids would seem well justified to reduce the time required to achieve this goal. Unfortunately, little is known concerning the androgenic response of the small number of genotypes with known partial FHB resistance. To make the best use of such FHB resistance donors in a barley improvement program, we first characterized the FHB resistance of eight reported FHB resistance sources (Chevron, Gobernadora, Seijo II, Shyri, Svanhals, Zhedar I, F104-250-9 and C97-21-38-3) in our own FHB nursery in Quebec City (QC, Canada). In parallel, we assessed the androgenic response of these same eight lines with that of three cultivars (ACCA, Léger and Cadette) of known androgenic response. Finally, the androgenic response of F1 hybrids involving some of these genotypes used as parents was measured and compared to that of the parental genotypes. Very large and significant differences were observed in the number of green plants produced by the different accessions and F1s. Although anther culture seemed very promising for some accessions, for others, the androgenic response was so low that a conventional approach would seem more appropriate.
Similar content being viewed by others
Abbreviations
- FHB:
-
Fusarium head blight
- DH:
-
Doubled haploid
- PDA:
-
Potato Dextrose Agar
References
Bai G, Shaner G (2004) Management and resistance in wheat and barley to Fusarium head blight. Annu Rev Phytopathol 42:135–161
Bourdages JV, Marchand S, Rioux S, Belzile FJ (2006) Diversity and prevalence of Fusarium species from Quebec barley fields. Can J Plant Pathol 28:1–7
Buerstmayr H, Legzdina L, Steiner B, Lemmens M (2004) Variation for resistance to Fusarium head blight in spring barley. Euphytica 137:279–290
Campbell KAG, Lipps PE (1998) Allocation of resources: sources of variation in Fusarium head blight screening nurseries. Phytopathology 88:1078–1086
Cappellini RA, Peterson JN (1965) Macroconidium formation in submerged cultures by non-sporulating strains of Gibberella zeae. Mycologia 57:962–966
Caredda S, Clément C (1999) Androgenesis and albinism in Poaceae: influence of genotype and carbohydrates. In: Clément C, Pacini E, Audran JC (eds) Anther and pollen: from biology to biotechnology. Springer, Heidelberg, pp 211–228
Caredda S, Doncoeur C, Devaux P, Sangwan RS, Clément C (2000) Plastid differentiation during androgenesis in albino and non-albino producing cultivars of barley (Hordeum vulgare L.). Sex Plant Reprod 13:95–104
Castillo AM, Cistué L, Romagosa I, Vallés MP (2001) Low responsiveness of six-rowed genotypes to androgenesis in barley does not have apleiotropic basis. Genome 44:936–940
Cho MJ, Jiang W, Lemaux PG (1998) Transformation of recalcitrant barley cultivars though improvement of regenerability and decreased albinism. Plant Sci 138:229–244
Dallaire C (2004) Caractérisation d’une population de lignées haploïdes doublées d’orge (Hordeum vulgare L.) en ségrégation pour la résistance à la fusariose de l’épi (Fusarium graminearum Schwabe). Mémoire de maîtrise. Université Laval, Québec, QC
Day A, Ellis THN (1985) Deleted forms of plastid DNA in albino plants from cereal anther culture. Curr Gentics 9:671–678
de la Pena RC, Smith KP, Capettini F, Muehlbauer GJ, Gallo-Meagher M, Dill-Macky R, Somers DA, Rasmusson DC (1999) Quantitative trait loci associated with resistance to Fusarium head blight and kernel discoloration in barley. Theor Appl Genet 99:561–569
Eudes F, Amundsen E (2005) Isolated microspore culture of Canadian 6x triticale cultivars. Plant Cell Tissue Organ Cult 82:233–241
Foroughi-Wehr B, Mix G, Gaul H, Wilson HM (1976) Plant production from cultured anthers of Hordeum vulgare L. Z Pflanzenzüchtg 77:198–204
Foroughi-Wehr B, Friedt W, Wenzel G (1982) On the genetic improvement of androgenetic haploid formation in Hordeum vulgare L. Theor Appl Genet 62:233–239
Foroughi-Wehr B, Friedt W, Wenzel G (1984) Rapid production of recombinant barley yellow mosaic virus resistant Hordeum vulgare lines by anther culture. Theor Appl Genet 67:377–382
Gerlach W, Nirenberg H (1982) The genus Fusarium—a pictorial atlas. Mitteilungen aus der Biologischen Bundesanstalt für and- und Forstwirtschaft Berlin-Dahlem 209:1–406
Hunter CP (1987) Plant regeneration method. European Patent Application N°87200773.7
Jähne A, Lörz H (1995) Cereal microspore culture. Plant Sci 109:1–12
Kasha KJ, Kao KN (1970) Hight frequency haploid production in barley (Hordeum vulgare L.). Nature 225:874–876
Kol B (1996) Haplodiploidisation d’hybrides interspécifiques ou intergénériques de blé. M.Sc. thesis, Université Laval, QC, Canada
Laroche L (1997) Influence de la concentration des composantes du milieu au début de la phase d’induction en culture in vitro d’anthères d’orge. M.Sc. thesis, Université Laval, QC, Canada
Larsen ET, Tuvesson IKD, Anderson SB (1991) Nuclear genes affecting percentage of green plants in barley (Hordeum vulgare L.). Theor Appl Genet 82:417–420
Luckett DJ, Smithard RA (1992) Doubled haploid production by anther culture for australian barley breeding. Aust J Agric Res 43:67–78
Ma Z, Steffenson BJ, Prom LK, Lapitan NLV (2000) Map** of quantitative trait loci for Fusarium head blight resistance in barley. Phytopathology 90:1079–1088
Manninen OM (2000) Associations between anther-culture response and molecular markers on chromosomes 2H, 3H and 4H of barley (Hordeum vulgare L.). Theor Appl Genet 100:57–62
Miles RM, Wilcoxson RD, Rasmusson DC (1989) Inheritance of resistance to kernel discoloration of barley. Plant Dis 73:711–715
Mesfin A, Smith KP, Dill-Macky R, Evans CK, Waugh R, Gustus CD, Muehlbauer GJ (2003) Quantitative trait loci for Fusarium head blight resistance in barley detected in tow-rowed by six-rowed population. Crop Sci 43:307–318
Miller JD, Culley J, Fraser K, et al (1998) Effect of tillage practices on Fusarium head blight of wheat. Can J Plant Sci 20:95–103
Nash SN, Snyder WC (1962) Quantitative estimations by plate counts of propagules of the bean rot Fusarium in field soils. Phytopathology 52:567–572
Nelson PE, Toussoun TA, Marasas WFO (1983) Fusarium species: an illustrated manual for identification. Pennsylvania State University, University Pack, PA, USA
Olsen FL (1991) Isolation and cultivation of embryogenic microspores from barley (Hordeum vulgare L.). Hereditas 115:255–266
Ouédraogo JT, St-Pierre CA, Collin J, Rioux S, Comeau A (1998) Effect of amino acids, growth regulators and genotype on androgenesis in barley. Plant Cell Tissue Organ Cult 53:59–66
Parry DW, Jenksinson P, McLeod L (1995) Fusarium ear blight (scab) in small grain cereals- a review. Plant Pathol (Oxford) 44:207–238
Prom LK, Steffenson BJ, Salas B, Fetch JR, TG, Casper HH (1996) Evaluation of selected barley accessions for resistance to Fusarium head blight and deoxynivalenol concentration. In: Scoles G, Rossnagel B (eds) Proceedings of the 5th international oat conference and the 7th international Barley genetics symposium. University Extension Press University of Saskatchewan, Saskatoon, SK, Canada, pp 764–766
Rioux S (2003) Fusarium head blight assessments in barley lines after inoculation with Fusarium graminearum and Fusarium sporotrichioides. In: Proceedings of the 3rd Canadian workshop on Fusarium head blight/Colloque Canadien sur la Fusariose, Winnipeg, Manitoba. December 9th–12th
Rudd JC, Horsley RD, McKendry AL, Elias EM (2001) Host plant resistance genes for Fusarium head blight: sources, mechanisms and utility in conventional breeding systems. Crop Sci 41:620–627
SAS Institute Inc. (1998) SAS/STAT™ user’s guide. Release 6.03 (ed) SAS Institue, Cary, NC, USA
Seifert K (1996) Fusarium interactive Key [online]. Agriculture and Agri-Food Canada. Available from http://www.ctu.edu.vn/colleges/agri/gtrinh/fuskey.pdf [accessed 8 November 2006]
Shands RG (1939) Chevron, a barley variety resistant to stem rust and other diseases. Phytopathology 29:209–211
Steffenson BJ, Prom LK, Salas B, Fetch TG, Wesenberg DM, Bockleman HE (1996) Severity of Fusarium head blight and concentrations of deoxynivalenol in near-isogenic lines of barley differing for several agronomic characters. In: Slinkard A et al (eds) Proceedings of the 7th international barley genetics symposium. University Extension press, Saskatoon, Saskatchewan, pp 774–776
Takeda K (1990) Selection response and parent-offspring correlation of the resistance to Fusarium head blight in barley. Jpn J Breed 40:91–101
Takeda K, Heta H (1989) Establishing the testing method and seach for the resistant varieties to Fusarium head blight in Japan. Jpn J Breed 39:203–216
Taniguchi M, Enomoto S, Komatsuda T, Kakajima K, Ohyama K (1991) Varietal differences in the ability of callus formation and plant regeneration from mature embryo in barley (Hordeum vulgare L.). Jpn J Breed 41:571–579
Trottier MC, Collin J, Comeau A (1993) Comparison of media for their aptitude in wheat anther culture. Plant Cell Tissue Organ Cult 35:59–65
Tuvesson IKD, Pedersen S, Andersen SB (1989) Nuclear genes affecting albinism in wheat (Triticum aestivum L.) anther culture. Theor Appl Genet 78:879–883
Urrea CA, Horlsey RD, Steffenson JB, Schwarz PB (2002) Heritability of Fusarium head blight resistance and deoxynivalenol accumulation from barley accession CIho 4196. Crop Sci 42:1404–1408
Wheatley WG, Marsolais AA, Kasha KJ (1986) Microspore growth and anther staging in barley anther culture. Plant Cell 5:47–49
Zadoks JC, Chang TT, Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Res 14:415–421
Acknowledgements
The authors are indebted to Mr. Martin Lacroix, Mr Marco Bussière and Mr D. Marois for their technical assistance. This project was funded by Semences Prograin Inc., the Conseil de recherches en pêche et en agrioalimentaire du Québec (CORPAQ) and La Coopérative Fédérée Inc.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by J.R. Liu.
Rights and permissions
About this article
Cite this article
Marchand, S., Fonquerne, G., Clermont, I. et al. Androgenic response of barley accessions and F1s with Fusarium head blight resistance. Plant Cell Rep 27, 443–451 (2008). https://doi.org/10.1007/s00299-007-0477-6
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00299-007-0477-6