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Successful genetic transformation of Chinese cabbage using phosphomannose isomerase as a selection marker

  • Genetic Transformation and Hybridization
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Abstract

A mannose selection system was adapted for use in the Agrobacterium-mediated transformation of Chinese cabbage. This system makes use of the pmi gene that encodes phosphomannose isomerase, which converts mannose-6-phosphate to fructose-6-phosphate. Hypocotyl explants from 4–5-day-old seedlings of Chinese cabbage inbred lines were pre-cultured for 2–3 days and then infected with Agrobacterium. Two genes (l-guluno-γ-lactone oxidase, GLOase, and jasmonic methyl transferase, JMT) were transformed into Chinese cabbage using the transformation procedure developed in this study. We found that supplementing the media with 7 g l−1 mannose and 2% sucrose provides the necessary conditions for the selection of transformed plants from nontransformed plants. The transformation rates were 1.4% for GLOase and 3.0% for JMT, respectively. The Southern blot analysis revealed that several independent transformants (T 0) were obtained from each transgene. Three different inbred lines were transformed, and most of the T 1 plants had normal phenotypes. The transformation method presented here for Chinese cabbage using mannose selection is efficient and reproducible, and it can be useful to introduce a desirable gene(s) into commercially useful inbred lines of Chinese cabbage.

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Abbreviations

BA :

Benzyl-aminopurine

pmi :

Phosphomannose isomerase

NAA :

Naphthaleneacetic acid

MS:

Murashige and Skoog

JMT :

Jasmonic methyl transferase

GLOase :

l-guluno-γ-lactone oxidase

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Acknowledgement

This research was supported by a grant (CG2242) from the Crop Functional Genomics Center of the 21st Century Frontier Research Program funded by the Ministry of the Science and Technology of the Republic of Korea.

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Authors and Affiliations

  1. Department of Plant Resources, Sangju National University, Sangju, Gyeongbuk, Korea

    Byung-Whan Min

  2. Biotechnology Institute, Nong Woo Bio Co. Ltd., Yeoju, Gyeonggi, Korea

    Yi-Nam Cho, Min-Jung Song, Tae-Kyung Noh, Bong-Kyu Kim, Won-Ki Chae, Young-Soo Park & Chee-Hark Harn

  3. School of Agricultural Biotechnology, Seoul National University, Seoul, Korea

    Yang-Do Choi

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  1. Byung-Whan Min
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  2. Yi-Nam Cho
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Correspondence to Chee-Hark Harn.

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Communicated by J. R. Liu

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Min, BW., Cho, YN., Song, MJ. et al. Successful genetic transformation of Chinese cabbage using phosphomannose isomerase as a selection marker. Plant Cell Rep 26, 337–344 (2007). https://doi.org/10.1007/s00299-006-0247-x

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  • DOI: https://doi.org/10.1007/s00299-006-0247-x

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