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Isolation of a maize beta-glucosidase gene promoter and characterization of its activity in transgenic tobacco

  • Genetic Transformation and Hybridization
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Abstract

The β-glucosidase gene of maize (ZmGLU1) was suggested to hydrolyze cytokinin-conjugate and release free cytokinin during plant growth and development. A clone containing the upstream region of ZmGLU1 was isolated and sequenced from a maize genomic library. The full-length ZmGLU1 promoter and a series of its 5′ deletions were fused to the beta-glucuronidase (GUS) reporter gene and transferred into tobacco. The GUS activity of transgenic plants was assayed at various developmental stages. The results showed that ZmGLU1 promoter-driven GUS gene had the highest expression level in the roots and that the expression of GUS gene declined during seed maturation and down to the lowest level in mature seeds. The ZmGLU1 promoter-driven GUS expression increased during seed germination, reaching a peak on day 11. The results also showed that this promoter could be inhibited by 6-BA, trans-zeatin, and NAA, but was not affected by GA3, ABA, SA, cold, salt, drought, and submergence treatments. The histochemical staining revealed that GUS activity was located in vigorous cell division zones with dominant staining associated with vascular tissues. Deletion analysis showed that the promoter contained a putative leaf-specific and stem-specific negative regulative element and two putative enhancers.

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Abbreviations

ABA:

Abscisic acid

BA:

Benzylaminopurine

CaMV:

Cauliflower mosaic virus

cef:

Cefotaxim

GA:

Gibberellin

IAA:

Indole-3-acetic acid

NAA:

Naphthalene-acetic acid

ppt:

Phosphinothricin

SA:

Salicylic acid

ZmGLU1P :

ZmGLU1 promoter

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Acknowledgement

This research was supported by China High-tech program (863) and the Cultivation Fund of the Key Scientific and Technical Innovation Project, Ministry of Education of China (No.: 705009).

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Authors and Affiliations

  1. State Key Laboratory for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, Bei**g, 100094, China

    Riliang Gu, Li Zhao, Ying Zhang, ** Chen, Juan Bao, **feng Zhao, Zhangying Wang, Junjie Fu, Tingsong Liu, Jianhua Wang & Guoying Wang

  2. Institute of Crop Sciences, CAAS, Bei**g, 100081, China

    Guoying Wang

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  1. Riliang Gu
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  2. Li Zhao
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Correspondence to Guoying Wang.

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Communicated by P. Puigdoménech

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Gu, R., Zhao, L., Zhang, Y. et al. Isolation of a maize beta-glucosidase gene promoter and characterization of its activity in transgenic tobacco. Plant Cell Rep 25, 1157–1165 (2006). https://doi.org/10.1007/s00299-006-0177-7

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  • DOI: https://doi.org/10.1007/s00299-006-0177-7

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