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FPF1 transgene leads to altered flowering time and root development in rice

  • Genetic Transformation and Hybridization
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Abstract

AtFPF1 (FLOWERING PROMOTING FACTOR 1) is a gene that promotes flowering in Arabidopsis. An expression vector containing AtFPF1 driven by a Ubi-1 promoter was constructed. The gene was introduced into rice callus by Agrobacterium-mediated transformation and fertile plants were obtained. The presence of AtFPF1 in rice plants was confirmed by PCR, Southern and Northern blot analyses, as well as by β-glucuronidase assay. The results showed that, as in Arabidopsis, AtFPF1 reduced flowering time in rice. Furthermore, introduction of AtFPF1 enhanced adventitious root formation but inhibited root growth in rice during the seedling stage. The results suggest that AtFPF1 promotes flowering time in both dicots and monocots, and plays a role in the initiation of adventitious roots in rice.

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Abbreviations

6-BA:

6-Benzyladenine

GUS:

β-Glucuronidase

Hyg:

Hygromycin

KT:

Kinetin

NAA:

α-Napthylacetic acid

ZT:

Zeatin

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Acknowledgements

The authors are grateful to Dr. S. Melzer, Swiss Federal Institute of Technology, Institute for Plant Sciences, Zurich, Switzerland, for his kind gift of the plasmid with AtFPF1. We also thank Dr. C.B. Chen, Penn State University, Dr. L.N. Tian, Agriculture & Agri-food Canada, and Dr. C Larue, University of Missouri for their critical reading of the manuscript. This project was supported by the Major State Basic Research Program of P.R. China (G19990116), partially by the National Nature Science Foundation of China (NSFC, 30270143), and the Innovation Grant of CAS, as well as the State High-Tech Project (2001AA222281).

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Authors and Affiliations

  1. Research Center for Molecular & Developmental Biology, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Chinese Academy of Sciences, Bei**g, 100093, China

    Ming-Li Xu, Jia-Fu Jiang, Lei Ge, Yun-Yuan Xu, Hui Chen, Yuan Zhao & Kang Chong

  2. College of Life Science, Lanzhou University, Lanzhou, 730000, China

    Ming-Li Xu, Yu-Rong Bi & Jiang-Qi Wen

  3. Department of Biology, Shanxi Normal University, Yuncheng, Shanxi, China

    Hui Chen

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  1. Ming-Li Xu
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  2. Jia-Fu Jiang
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  3. Lei Ge
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  4. Yun-Yuan Xu
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  5. Hui Chen
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  6. Yuan Zhao
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  9. Kang Chong
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Corresponding author

Correspondence to Kang Chong.

Additional information

Communicated by P. Lakshmanan

Ming-Li Xu and Jia-Fu Jiang contributed equally to this work

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Xu, ML., Jiang, JF., Ge, L. et al. FPF1 transgene leads to altered flowering time and root development in rice. Plant Cell Rep 24, 79–85 (2005). https://doi.org/10.1007/s00299-004-0906-8

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