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Effects of Soluble Phosphate on Phosphate-Solubilizing Characteristics and Expression of gcd Gene in Pseudomonas frederiksbergensis JW-SD2

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Abstract

Phosphate-solubilizing bacteria have the ability of solubilizing mineral phosphate in soil and promoting growth of plants, but the activity of phosphate solubilization is influenced by exogenous soluble phosphate. In the present study, the effects of soluble phosphate on the activity of phosphate solubilization, acidification of media, growth, and organic acid secretion of phosphate-solubilizing bacterium Pseudomonas frederiksbergensis JW-SD2 were investigated under six levels of soluble phosphate conditions. The activity of phosphate solubilization decreased with the increase of soluble phosphate concentration, accompanying with the increase of media pH. However, the growth was promoted by adding soluble phosphate. Production of gluconic, tartaric, and oxalic acids by the strain was reduced with the increase of concentration of soluble phosphate, while acetic and pyruvic acids showed a remarkable increase. Gluconic acid predominantly produced by the strain at low levels of soluble phosphate showed that this acid was the most efficient organic acid in phosphate solubilization. Pyrroloquinoline quinone-glucose dehydrogenase gene gcd (pg5SD2) was cloned from the strain, and the expressions of pg5SD2 gene were repressed gradually with the increase of concentration of soluble phosphate. The soluble phosphate regulating the transcription of the gcd gene is speculated to underlie the regulation of the secretion of gluconic acid and subsequently the regulation of the activity of phosphate solubilization. Future research needs to consider a molecular engineering strategy to reduce the sensitivity of PSB strain to soluble phosphate via modification of the regulatory mechanism of gcd gene, which could improve the scope of PSB strains’ application.

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Acknowledgments

This research was supported by the Chinese Special Research Program for Forestry Sectors Beneficial to Public (201004061), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and Innovation Plan for Graduate Students of Jiangsu, China (CXLX12_0544). The authors express their gratitude to Dr. De-Wei Li, The Connecticut Agricultural Experiment Station for reviewing the manuscript.

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  1. Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nan**g Forestry University, Nan**g, China

    Qingwei Zeng, **aoqin Wu & **nyi Wen

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  1. Qingwei Zeng
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  2. **aoqin Wu
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Correspondence to **aoqin Wu.

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Zeng, Q., Wu, X. & Wen, X. Effects of Soluble Phosphate on Phosphate-Solubilizing Characteristics and Expression of gcd Gene in Pseudomonas frederiksbergensis JW-SD2. Curr Microbiol 72, 198–206 (2016). https://doi.org/10.1007/s00284-015-0938-z

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  • DOI: https://doi.org/10.1007/s00284-015-0938-z

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