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Striae Distensae: In Vitro Study and Assessment of Combined Treatment With Sodium Ascorbate and Platelet-Rich Plasma on Fibroblasts

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  • Basic Science/Experimental
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Abstract

Introduction

Striae distensae (SD) appear clinically as parallel striae, lying perpendicular to the tension lines of the skin. SD evolve into two clinical phases, an initial inflammatory phase in which they are called “striae rubrae” (SR) and a chronic phase in which they are called striae albae (SA). Fibroblasts seem to play a key role in the pathogenesis of stretch marks. This study was aimed at describing and analyzing stretch marks-derived fibroblasts (SMF), the differences between SR- and SA-derived fibroblasts (SRF, SAF), testing two treatments in vitro (sodium ascorbate and PrP) on SAF.

Material and Methods

To characterize the SMF, the expression of alpha smooth muscle actin (alpha SMA) was investigated. Type I collagen expression was measured in SAF, before and after adding different PrP concentrations and sodium ascorbate in the culture medium. Results were processed through statistical analysis models using the Student’s t-test.

Results

A significant increase in alpha SMA (P <0.001) was observed in SRF. SAF treated with PrP and sodium ascorbate showed a resumption of their metabolic activity by an increase in collagen type I production and cell proliferation. After 24 h of incubation with PrP 1% and PrP 5% + sodium ascorbate, cell viability was increased by 140% and 151% and by 156 and 178% after 48 h, respectively, compared to the control.

Conclusion

Our study shows that a biologically mediated improvement in SMF metabolic activity is possible. Our promising results require further trials to be able to confirm the reproducibility of this combined treatment, particularly in vivo.

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Authors and Affiliations

  1. Department of Plastic, Reconstructive and Maxillo facial Surgery, Henri Mondor Hospital, University Paris XII, 51 Avenue du Maréchal de Lattre de Tassigny, 94000, Créteil, France

    Simone La Padula, Barbara Hersant, Chiara Pizza, Concetta Errico & Jean Paul Meningaud

  2. Biopredic International, Parc d’activité de la Bretèche Bâtiment A4, 35760, Saint Grégoire, France

    Christophe Chesné, Agnes Jamin & Ismail Ben Mosbah

  3. Department of Plastic and Reconstructive Surgery, Università Campus Biomedico di Roma, Via Alvaro del Portillo, 21, 00128, Roma, Italy

    Simone La Padula & Paolo Persichetti

  4. IMRB - Institut Mondor de Recherche Biomédicale, 51 Avenue du Maréchal de Lattre de Tassigny, 94000, Créteil, France

    Ismail Ben Mosbah

  5. Department of Plastic and Reconstructive Surgery, Università degli studi di Napoli Federico II, Via Pansini 5, 80131, Napoli, Italy

    Simone La Padula, Francesco D’Andrea & Umberto Rega

  6. 50 rue Saint Sébastien, Paris, 75011, France

    Simone La Padula

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  1. Simone La Padula
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Correspondence to Simone La Padula.

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The authors declare that they have no conflict of interest.

Ethical Approval

Ethical approval was given, by FRENCH institutional committee, and the relevant judgement’s reference number is 2020-A01250-39.

Human and Animal Participants

All procedures in the study involving human participants have been performed in accordance with the ethical standards of institutional and/or national research committees and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

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La Padula, S., Hersant, B., Pizza, C. et al. Striae Distensae: In Vitro Study and Assessment of Combined Treatment With Sodium Ascorbate and Platelet-Rich Plasma on Fibroblasts. Aesth Plast Surg 45, 1282–1293 (2021). https://doi.org/10.1007/s00266-020-02100-7

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  • DOI: https://doi.org/10.1007/s00266-020-02100-7

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