Abstract
Trichoderma viride has a wide range of applications in plant growth promotion, biological control, cellulase production, and biomass utilization. Salinity is a major limitation to Trichoderma strains in the natural environment and fermentation environment, and to improve the adaptability of Trichoderma to salt stress is of great significance to its applications in industry and agriculture. Histone acetylation plays important roles in the regulation of physiological and biochemical processes including various stress responses. GCN5 is the most representative histone acetylase, which plays vital roles in chromatin remodeling of promoters to facilitate the transcription activation. In this paper, we identified a GCN5-encoding gene TvGCN5 in T. viride Tv-1511, and characterized the function and regulating mechanism of TvGCN5-mediated acetylation of histone H3 in the salt adoption of Tv-1511, by constructions of the deletion mutants (Tv-1511-△GCN5) and overexpression mutants (Tv-1511-GCN5-OE) of TvGCN5. Results showed that compared with wild-type Tv-1511, the over-expression of TvGCN5 resulted in the longer mycelia diameter and more biomass under salt stress. Furthermore, Tv-1511-△GCN5 strains obtained the improved sodium (Na+) compartmentation and antioxidant capacity by upregulating the transcriptional levels of genes encoding PM H+-ATPase, vacuolar H+-ATPase, and antioxidant enzymes. Notably, the changes in the transcriptional expressions of these genes are tightly modulated by the TvGCN5-mediated acetylated level of histone H3 in their promoter regions. In all, these results reveal that TvGCN5 plays an important role in stress tolerance of T. viride Tv-1511, and provides potential insight to facilitate the application of epigenetic modulation in the expanding utilization of Trichoderma.
Key points
• Overexpresison of TvGCN5 improves the adoption of T. viride Tv-1511 to salt stress by increasing acetylation level of histone H3 on the promoter regions of sodium-transport and antioxidant-related genes, at H3K9ac, H3K14ac, H3K23ac, and H3K27ac.
• Overexprsison of TvGCN5 enhances the ion transport and compartmentation capacity by upregulating the expressions and activities of PM and vacuolar H+-ATPase to tolerate salt stress.
• Overexprsison of TvGCN5 promotes the antioxidant capacity by increasing the expressions and activities of antioxidant enzymes in response to salt stress.
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This research is supported by the National Natural Science Foundation of China (21607169; 41977125; 41907033; 21254356), the National Natural Science Foundation of Shandong Province (ZR202102270296), the Natural Science Foundation of Guangdong Province (2019A1515011948), the Youth Science Funds of Shandong Academy of Sciences (2020QN0019), the Program for Innovation Team in the University of **an (2019GXRC033), the Key R&D Program of Yantai (2019ZDCX016), and the Special Science and Technology Project of Rizhao (2019CXZX2205).
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ZL conceived the project; ZL and ZL designed the experiments; ZL, HZ, CJC, ZZ, JC, and KG carried out the experiments; ZL and HZ analyzed the data; ZL, HZ, and ZL wrote the article. All authors read and approved the final manuscript.
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Li, Z., Zhang, H., Cai, C. et al. Histone acetyltransferase GCN5-mediated lysine acetylation modulates salt stress aadaption of Trichoderma. Appl Microbiol Biotechnol 106, 3033–3049 (2022). https://doi.org/10.1007/s00253-022-11897-z
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DOI: https://doi.org/10.1007/s00253-022-11897-z