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Optimization of enzymatic hydrolysis of pretreated rice straw and ethanol production

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Abstract

Cellulase, Tween 80, and β-glucosidase loading were studied and optimized by response surface methodology to improve saccharification. Microwave alkali-pretreated rice straw used as substrate for onsite enzyme production by Aspergillus heteromorphus and Trichoderma reesei. The highest enzymatic hydrolysis (84%) was obtained from rice straw at crude enzyme loading of 10 FPU/gds of cellulase, 0.15% Tween 80, and 100 international unit/g dry solids of β-glucosidase activities. Enzymatic hydrolyzate of pretreated rice straw was used for ethanol production by Saccharomyces cerevisiae, Scheffersomyces stipitis, and by co-culture of both. The yield of ethanol was 0.50, 0.47, and 0.48 gp/gs by S. cerevisiae, S. stipitis, and by co-culture, respectively, using pretreated rice straw hydrolyzate. The co-culture of S. cerevisiae and S. stipitis produced 25% more ethanol than S. cerevisiae alone and 31% more ethanol than S. stipitis alone. During anaerobic fermentation 65.08, 36.45, and 50.31 μmol/ml CO2 released by S. cerevisiae, S. stipitis, and by co-culture, respectively. The data indicated that saccharification efficiency using optimized crude enzyme cocktail was good, and enzymatic hydrolyzate could be fermented to produce ethanol.

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Acknowledgments

The authors acknowledge the financial assistance to Ms. Anita Singh by CSIR, New Delhi in the form of Senior Research Fellowship and University Grant Commission, New Delhi for providing financial support under the Major Research Project scheme F-33-144/2007(SR).

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  1. Department of Environmental Science and Engineering, Guru Jambheshwar University of Science and Technology, Hisar, 125001, Haryana, India

    Anita Singh & Narsi R. Bishnoi

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  1. Anita Singh
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  2. Narsi R. Bishnoi
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Correspondence to Anita Singh or Narsi R. Bishnoi.

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Singh, A., Bishnoi, N.R. Optimization of enzymatic hydrolysis of pretreated rice straw and ethanol production. Appl Microbiol Biotechnol 93, 1785–1793 (2012). https://doi.org/10.1007/s00253-012-3870-1

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  • DOI: https://doi.org/10.1007/s00253-012-3870-1

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