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Cell adaptation to solvent, substrate and product: a successful strategy to overcome product inhibition in a bioconversion system

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Abstract

Carvone has previously been found to highly inhibit its own production at concentrations above 50 mM during conversion of a diastereomeric mixture of (−)-carveol by whole cells of Rhodococcus erythropolis. Adaptation of the cells to the presence of increasing concentrations of carveol and carvone in n-dodecane prior to biotransformation proved successful in overcoming carvone inhibition. By adapting R. erythropolis cells for 197 h, an 8.3-fold increase in carvone production rate compared to non-adapted cells was achieved in an air-driven column reactor. After an incubation period of 268 h, a final carvone concentration of 1.03 M could be attained, together with high productivity [0.19 mg carvone h−1 (ml organic phase)−1] and high yield (0.96 g carvone g carveol−1).

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Acknowledgements

This study was supported by a Ph.D. (PRAXIS XXI/BD/21574/99) and a post-doctoral grant (SFRH/BPD/14426/2003) awarded to Carla da C. C. R. de Carvalho by Fundação para a Ciência e a Tecnologia, Portugal and by a SOCRATES- ERASMUS grant awarded to Alessandro Poretti.

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Authors and Affiliations

  1. Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001, Lisbon, Portugal

    Carla C. C. R. de Carvalho, Alessandro Poretti & M. Manuela R. da Fonseca

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  1. Carla C. C. R. de Carvalho
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  2. Alessandro Poretti
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  3. M. Manuela R. da Fonseca
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Correspondence to Carla C. C. R. de Carvalho.

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de Carvalho, C.C.C.R., Poretti, A. & da Fonseca, M.M.R. Cell adaptation to solvent, substrate and product: a successful strategy to overcome product inhibition in a bioconversion system. Appl Microbiol Biotechnol 69, 268–275 (2005). https://doi.org/10.1007/s00253-005-1967-5

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  • DOI: https://doi.org/10.1007/s00253-005-1967-5

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