Abstract
Redox potential was used to develop a stationary-phase fermentation of Candida tropicalis that resulted in non-growth conditions with a limited decline in cell viability, a xylitol yield of 0.87 g g−1 (95% of the theoretical value), and a high maximum specific production rate (0.67 g g−1 h−1). A redox potential of 100 mV was found to be optimum for xylitol production over the range 0–150 mV. A shift from ethanol to xylitol production occurred when the redox potential was reduced from 50 mV to 100 mV as cumulative ethanol (Y ethanol) decreased from 0.34 g g−1 to 0.025 g g−1 and Y xylitol increased from 0.15 g g−1 to 0.87 g g−1 (α=0.05). Reducing the redox potential to 150 mV did not improve the fermentation. Instead, the xylitol yield and productivity decreased to 0.63 g g−1 and 0.58 g g−1 h−1 respectively and cell viability declined. The viable, stationary-phase fermentation could be used to develop a continuous fermentation process, significantly increasing volumetric productivity and reducing downstream separation costs, potentially by the use of a membrane cell-recycle reactor.
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References
Barbosa MFS, Medeiros MB, Mancilha IM de, Schneider H, Lee H (1988) Screening for yeasts for production xylitol from some factors which affect xylitol yield in Candida guillermondi. J Ind Microbiol 3:241–251
Berovic M (1999) Scale-up of citric acid fermentation by redox potential control. Biotechnol Bioeng 64:552–557
Bruinenburg PM, Bot PHM de, Dijken JP van, Scheffers, WA (1983) The role of redox balances in the anaerobic fermentation of xylose by yeasts. Appl Microbiol Biotechnol 18:287–292
Choi J-H, Moon K-H, Ryu Y-W, Seo J-H (2000) Production of xylitol in cell recycle fermentations of Candida tropicalis. Biotechnol Lett 22:1625–1628
Chung IS, Lee YY (1986) Effect of oxygen and redox potential on d-xylose fermentation by non-growing cells of Pachysolen tannophilus. Enzyme Microb Technol 8:503–507
Hashimoto SI, Katsumata R (1999) Mechanism of alanine hyperproduction by Arthrobacter oxydans HAP-1: metabolic shift to fermentation under nongrowth aerobic conditions. Appl Environ Microbiol 65:2781–2783
Kastner JR, Roberts RS (1990) Simultaneous fermentation of d-xylose and glucose by Candida shehatae. Biotechnol Lett 12:57–60
Kastner JR, Ahmad M, Jones WJ, Roberts RS (1992) Viability of Candida shehatae in d-xylose fermentations with added ethanol. Biotechnol Bioeng 40:1282–1285
Kastner JR, Roberts RS, Jones WJ (1999) Oxygen starvation induces cell death in d-xylose Candida shehatae fermentations, but not in glucose. Appl Microbiol Biotechnol 51:780–783
Kastner JR, Eiteman MA, Lee SA. (2001) Glucose repression of xylitol production in Candida tropicalis mixed-sugar fermentations. Biotechnol Lett 23:1663–1668
Kim EK, Roberts RS (1991) Rate equations for the vigorous stationary phase fermentation of citric acid by Saccharomyces lipolytica. Biotechnol Bioeng 37:985–988
Kim JH, Ryu YW, Seo JH (1999) Analysis and optimization of a two-substrate fermentation for xylitol production using Candida tropicalis. J Ind Microbiol Biotechnol 22:181–186
Kim JH, Han KC, Koh YH, Ryu YW, Seo JH (2002) Optimization of fed-batch fermentation for xylitol production Candida tropicalis. J Ind Microbiol Biotechnol 29:16–19
Oh DK, Kim SY (1998) Increase of xylitol yield by feeding xylose and glucose in Candida tropicalis. Appl Microbiol Biotechnol 50:419–425
Oh DK, Kim SY, Kim JH (1998) Increase of xylitol production rate by controlling redox potential in Candida parapsilosis. Biotechnol Bioeng 58:440–444
Radjai MK, Hatch RT, Cadman TW (1984) Optimisation of amino acid production by automatic self tuning digital control of redox potential. Biotechnol Bioeng Symp 14: 657–666
Rose AH, Harrison JS (1989) Metabolism and physiology of yeasts. (The yeasts, vol 3) Academic Press, New York
Schneider H (1989) Conversion of pentoses to ethanol by yeasts and fungi. Crit Rev Biotechnol 9:2–41
Shi NQ, Jeffries TW (1998) Anaerobic growth and improved fermentation of Pichia stipitis bearing a URA1 gene from Saccharomyces cerevisiae. Appl Microbiol Biotechnol 50:339–345
Von Weymarn N, Kiviharju K, Leisola M (2002) High-level production of d-mannitol with membrane cell-recycle bioreactor. J Ind Microbiol Biotechnol 29:44–49
Walton AZ, Stewart JD (2002) An Efficient enzymatic baeyer-villiger oxidation by engineered Escherichia coli cells under non-growing conditions. Biotechnol Prog 18:262–268
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This research was supported through the Traditional Industries, FoodPac Program (State of Georgia, USA).
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An erratum to this article can be found at http://dx.doi.org/10.1007/s00253-004-1583-9
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Kastner, J.R., Eiteman, M.A. & Lee, S.A. Effect of redox potential on stationary-phase xylitol fermentations using Candida tropicalis . Appl Microbiol Biotechnol 63, 96–100 (2003). https://doi.org/10.1007/s00253-003-1320-9
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DOI: https://doi.org/10.1007/s00253-003-1320-9