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The DY genes of the cattle MHC: expression and comparative analysis of an unusual class II MHC gene pair

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Abstract

The major histocompatibility complex of cattle (BoLA) contains the class II genes DYA and DIB which are transcribed with a dendritic cell restricted distribution. As part of the process to determine whether these genes have any functional significance, we demonstrate that they form a closely linked pair characteristic of other expressed class II MHC molecules. Accepted nomenclature convention suggests that BoLA-DIB should therefore be renamed BoLA-DYB. Analysis of the first full-length DYA and DYB transcripts revealed open reading frames with potential to translate 253 and 259 amino acid proteins, respectively. Comparative sequence analysis between the DY polypeptides and classical cattle, human and mouse class II MHC α and β polypeptide chains revealed 16 unique amino acid residues at positions predicted to form and line the putative peptide-binding region. Expression of tagged constructs demonstrates for the first time that the DY genes of cattle are capable of translating distinctive class II MHC α and β polypeptide chains.

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Acknowledgements

The authors wish to thank Dr. Bernadette Dutia for providing the sheep class II-specific monoclonal antibodies, the Clinical Division of the Moredun Institute for providing samples and supporting cannulation studies and the Wellcome Trust Centre for Research in Comparative Respiratory Medicine for providing laboratory facilities. All experiments comply with the regulatory requirements of the United Kingdom. K. Ballingall acknowledges the funding support of the Wellcome Trust through grant 065442.

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Authors and Affiliations

  1. Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, Easter Bush Veterinary Centre, The University of Edinburgh, Easter Bush, Roslin, Midlothian, EH25 9RG, UK

    Keith T. Ballingall & Declan J. McKeever

  2. Institute for Animal Health, Compton, Newbury, Berkshire, RG20 7NN, UK

    Shirley A. Ellis & Stephen D. Archibald

  3. The Centre for Tropical Veterinary Medicine, Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Easter Bush, Roslin, Midlothian, EH25 9RG, UK

    Niall D. MacHugh

  4. The Moredun Research Institute, Pentland Science Park, Bush Loan, Penicuik, Midlothian, EH26 OPZ, UK

    Declan J. McKeever

Authors
  1. Keith T. Ballingall
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  2. Shirley A. Ellis
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  3. Niall D. MacHugh
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  4. Stephen D. Archibald
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  5. Declan J. McKeever
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Corresponding author

Correspondence to Keith T. Ballingall.

Additional information

Nucleotide sequence data reported here have the following accession numbers: AJ580583 and AJ580584

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Ballingall, K.T., Ellis, S.A., MacHugh, N.D. et al. The DY genes of the cattle MHC: expression and comparative analysis of an unusual class II MHC gene pair. Immunogenetics 55, 748–755 (2004). https://doi.org/10.1007/s00251-004-0641-x

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  • DOI: https://doi.org/10.1007/s00251-004-0641-x

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