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Liquid-ordered microdomains in lipid rafts and plasma membrane of U-87 MG cells: a time-resolved fluorescence study

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Abstract

Lipid rafts, the functional microdomains in the cell membrane, are believed to exist as liquid-ordered (Lo) phase domains along with the liquid-disordered (Ld) phase of the bulk of the cell membranes. We have examined the lipid order in model and natural membranes by time-resolved fluorescence of trimethylammonium-1,6-diphenylhexatriene incorporated into the membranes. The lipid phases were discerned by the limiting anisotropy, rotational diffusion rate and distribution of the fluorescence lifetime. In dipalmitoylphosphatidylcholine (DPPC)-cholesterol mixtures the gel phase exhibited higher anisotropy and a two-fold slower rotational diffusion rate of the probe as compared to the Ld phase. On the other hand, the Lo phase exhibited higher limiting anisotropy but a rotational diffusion rate comparable to the Ld phase. The Ld and Lo phases elicited unimodal distribution of lifetimes with distinct mean values and their co-existence in phospholipid-cholesterol mixtures was reflected as a biphasic change in the width of the lifetime distribution. Global analysis of the lifetimes yielded a best fit with two lifetimes which were identical to those observed in single Lo or Ld phases, but their fractional contribution varied with cholesterol concentration. Attributing the shorter and longer lifetime components to the Ld and Lo phases, respectively, the extent of the Lo/Ld phase domains in the membranes was estimated by their fractional contribution to the fluorescence decay. In ternary mixtures of egg PC-gangliosides-cholesterol, the gangliosides induced heterogeneity in the membrane but the Ld phase prevailed. The Lo phase properties were observed only in the presence of cholesterol. Results obtained in the plasma membrane and detergent-resistant membrane fractions (DRMs) isolated from U-87 MG cells revealed that DRMs mainly possess the Lo phase; however, a substantially large proportion of plasma membrane also exists in the Lo phase. Our data show that, besides cholesterol, the membrane proteins play a significant role in the organization of lipid rafts and, furthermore, a considerable amount of heterogeneity is present among the lipid rafts.

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Abbreviations

DPPC:

dipalmitoylphosphatidylcholine

DRM:

detergent-resistant membrane fraction

EPC:

egg phosphatidylcholine

GSL:

glycosphingolipid

Ld :

liquid-disordered phase domains

Lo :

liquid-ordered phase domains

MBCD:

methyl-β-cyclodextrin

PBS:

phosphate buffered saline

PM:

plasma membrane

TMA-DPH:

trimethylammonium-1,6-diphenylhexatriene

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Acknowledgements

This work was supported by the Council of Scientific and Industrial Research, New Delhi, India.

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  1. Department of Biophysics, National Institute of Mental Health and Neurosciences, 560 029, Bangalore , India

    Mau Sinha, Sudha Mishra & Preeti G. Joshi

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  1. Mau Sinha
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  2. Sudha Mishra
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  3. Preeti G. Joshi
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Correspondence to Preeti G. Joshi.

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Sinha, M., Mishra, S. & Joshi, P.G. Liquid-ordered microdomains in lipid rafts and plasma membrane of U-87 MG cells: a time-resolved fluorescence study. Eur Biophys J 32, 381–391 (2003). https://doi.org/10.1007/s00249-003-0281-3

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