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Banana Fusarium Wilt Disease Incidence Is Influenced by Shifts of Soil Microbial Communities Under Different Monoculture Spans

  • Soil Microbiology
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Abstract

The continuous crop** of banana in the same field may result in a serious soil-borne Fusarium wilt disease and a severe yield decline, a phenomenon known as soil sickness. Although soil microorganisms play key roles in maintaining soil health, the alternations of soil microbial community and relationship between these changes and soil sickness under banana monoculture are still unclear. Bacterial and fungal communities in the soil samples collected from banana fields with different monoculture spans were profiled by sequencing of the 16S rRNA genes and internal transcribed spacer using the MiSeq platform to explore the relationship between banana monoculture and Fusarium wilt disease in the present study. The results showed that successive crop** of banana was significantly correlated with the Fusarium wilt disease incidence. Fungal communities responded more obviously and quickly to banana consecutive monoculture than bacterial community. Moreover, a higher fungal richness significantly correlated to a higher banana Fusarium wilt disease incidence but a lower yield. Banana fungal pathogenic genus of Fusarium and Phyllosticta were closely associated with banana yield depletion and disease aggravation. Potential biocontrol agents, such as Funneliformis, Mortierella, Flavobacterium, and Acidobacteria subgroups, exhibited a significant correlation to lower disease occurrence. Further networks analysis revealed that the number of functionally interrelated modules decreased, the composition shifted from bacteria- to fungi-dominated among these modules, and more resources-competitive interactions within networks were observed after banana long-term monoculture. Our results also showed that bacterial and fungal communities were mainly driven by soil organic matter. Overall, the findings indicated that the bacterial and fungal community structures altered significantly after banana long-term monoculture, and the fungal richness, abundance of Fusarium, interactions between and within bacteria and fungi in ecological networks, and soil organic matter were associated with banana soil-borne Fusarium wilt disease.

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Acknowledgements

We thank the banana orchard owner Mr. Yusheng Li for providing access to the experimental field and facilities. This research was supported by the National Key Basic Research Program of China (2015CB150500), Fundamental Research Funds for the Central Universities (KJQN201746), National Key Research and Development Program (2016YFD0800605 and 2016YFE0101100), National Natural Science Foundation of China (31601836, 31572212 and 31372142), Science and Technology Planning Project of Guangdong Province (2016B020202006), and China Postdoctoral Science Foundation (2016M590469).

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Authors and Affiliations

  1. Jiangsu Provincial Key Lab of Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, National Engineering Research Center for Organic-based Fertilizer, College of Resources and Environmental Sciences, Nan**g Agricultural University, Nan**g, 210095, China

    Zongzhuan Shen, Nana Lv, Chao Xue, **anfu Yuan, Rong Li & Qirong Shen

  2. College of Integrative Sciences and Arts, Julie Ann Wrigley Global Institute of Sustainability, Fundamental and Applied Microbiomics Institute, Arizona State University, Mesa, AZ, USA

    C. Ryan Penton

  3. Hainan key Laboratory for Sustainable Utilization of Tropical Bio-resources, College of Agriculture, Hainan University, Haikou, 570228, China

    Yunze Ruan

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  1. Zongzhuan Shen
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Correspondence to Rong Li.

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Shen, Z., Penton, C.R., Lv, N. et al. Banana Fusarium Wilt Disease Incidence Is Influenced by Shifts of Soil Microbial Communities Under Different Monoculture Spans. Microb Ecol 75, 739–750 (2018). https://doi.org/10.1007/s00248-017-1052-5

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