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Lipopeptides Produced by a Soil Bacillus Megaterium Strain

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Abstract

A soil microorganism identified as Bacillum megaterium was found to produce several antibiotics substances after growth for 20 h at 37°C in a mineral culture medium. Analysis both by electron spray ionization (ESI) and matrix-assisted laser desorption ionization—time of flight (MALDI-TOF) mass spectrometry (MS) identified these substances as lipopeptides. Predominant peaks at m/z 1,041 and m/z 1,065 revealed ions which are compatible with surfactins and lichenysins, respectively. Two other ions m/z 1,057 and m/z 1,464 were further studied by collision-induced dissociation (CID) unveiling an iturin A at the first and fengycins A and B at the second m/z peaks. The CID spectrum of the m/z 1,464 ion also suggests the existence of fengycins A and B variants in which Ile was changed to Val in the position 10 of the peptide moiety. Raw mixtures of all these compounds were also assayed for antibiotic features. The data enlighten the unusual diversity of the lipopeptide mixture produced by a sole Bacillus species.

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Acknowledgements

The authors are grateful to Fernanda Manso Prado, Izaura Nobuko Toma, and Sirlei Mendes de Oliveira for technical assistance. Financial support was from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, grant No 98/11480-1) and from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, grant No 473145/2006-5).

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Authors and Affiliations

  1. Departamento de Bioquimica, Instituto de Quimica, Universidade de São Paulo, São Paulo, SP, Brazil

    Manuel Troyano Pueyo, Ana Maria Carmona-Ribeiro & Paolo di Mascio

  2. Laboratório de Espectrometria de Massa, EMBRAPA, Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil

    Carlos Bloch Jr

  3. Av. Prof. Lineu Prestes 748, Bl. 10 Sup. room 1056, CEP 05508-0000, São Paulo, SP, Brazil

    Manuel Troyano Pueyo

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  1. Manuel Troyano Pueyo
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Correspondence to Manuel Troyano Pueyo.

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Pueyo, M.T., Bloch, C., Carmona-Ribeiro, A.M. et al. Lipopeptides Produced by a Soil Bacillus Megaterium Strain. Microb Ecol 57, 367–378 (2009). https://doi.org/10.1007/s00248-008-9464-x

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