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Effects of Culturing on the Population Structure of a Hyperthermophilic Virus

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Abstract

The existence of a culturing bias has long been known when sampling organisms from the environment. This bias underestimates microbial diversity and does not accurately reflect the most ecologically relevant species. Until now no study has examined the effects of culture bias on viral populations. We have employed culture-independent methods to assess the diversity of Sulfolobus spindle–shaped viruses (SSVs) from extremely hyperthermal environments. This diversity is then compared to the viral diversity of cultured samples. We detected a clear culturing bias between environmental samples and cultured isolates. This is the first study identifying a culture bias in a viral population.

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Acknowledgments

We thank Matthew Lavin and Mary Bateson for critically reading this manuscript. This research was conducted with permission from Yellowstone National Park research permit YELL-05090. This work is supported by grants from the National Science Foundation (MCB01322156) and the National Aeronautics and Space Administration (NAG5-8807).

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Authors and Affiliations

  1. Thermal Biology Institute, Montana State University, Bozeman, MT, 59717, USA

    J.C. Snyder, J. Spuhler, B. Wiedenheft, T. Douglas & M.J. Young

  2. Department of Biotechnologies, INEEL, Idaho Falls, ID, 83415, USA

    F.F. Roberto

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  1. J.C. Snyder
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  2. J. Spuhler
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  3. B. Wiedenheft
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  4. F.F. Roberto
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  5. T. Douglas
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  6. M.J. Young
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Correspondence to M.J. Young.

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Snyder, J., Spuhler, J., Wiedenheft, B. et al. Effects of Culturing on the Population Structure of a Hyperthermophilic Virus. Microb Ecol 48, 561–566 (2004). https://doi.org/10.1007/s00248-004-0246-9

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  • DOI: https://doi.org/10.1007/s00248-004-0246-9

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