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Riboflavin and Vitamin E Increase Brain Calcium and Antioxidants, and Microsomal Calcium-ATP-ase Values in Rat Headache Models Induced by Glyceryl Trinitrate

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Abstract

The essential use of riboflavin is the prevention of migraine headaches, although its effect on migraines is considered to be associated with the increased mitochondrial energy metabolism. Oxidative stress is also important in migraine pathophysiology. Vitamin E is a strong antioxidant in nature and its analgesic effect is not completely clear in migraines. The current study aimed to investigate the effects of glyceryl trinitrate (GTN)-sourced exogen nitric oxide (NO), in particular, and also riboflavin and/or vitamin E on involved in the headache model induced via GTN-sourced exogen NO on oxidative stress, total brain calcium levels, and microsomal membrane Ca2+-ATPase levels. GTN infusion is a reliable method to provoke migraine-like headaches in experimental animals and humans. GTN resulted in a significant increase in brain cortex and microsomal lipid peroxidation levels although brain calcium, vitamin A, vitamin C, and vitamin E, and brain microsomal-reduced glutathione (GSH), glutathione peroxidase (GSH-Px), and plasma-membrane Ca2+-ATPase values decreased through GTN. The lipid peroxidation, GSH, vitamin A, β-carotene, vitamin C, and vitamin E, and calcium concentrations, GSH-Px, and the Ca2+-ATPase activities were increased both by riboflavin and vitamin E treatments. Brain calcium and vitamin A concentrations increased through riboflavin only. In conclusion, riboflavin and vitamin E had a protective effect on the GTN-induced brain injury by inhibiting free radical production, regulation of calcium-dependent processes, and supporting the antioxidant redox system. However, the effects of vitamin E on the values seem more important than in riboflavin.

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Abbreviations

[Ca2+]i :

Intracellular Ca2+

CSD:

Cortical spreading depression

DMSO:

Dimethyl sulfoxide

GSH:

Reduced glutathione

GSH-Px:

Glutathione peroxidase

LP:

Lipid peroxidation

MMCA:

Microsomal membrane Ca2+-ATPase

NO:

Nitric oxide

PMCA:

Plasma-membrane Ca2+-ATPase

ROS:

Reactive oxygen species

VGCC:

Voltage-gated calcium channels

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Acknowledgments

The study was partially supported by the Scientific Research Project Unit of Suleyman Demirel University (BAP-1595-TU-07).

Conflict interest

None of the authors have any conflicts to disclose. All authors approved the final manuscript.

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Authors and Affiliations

  1. Department of Neurology, Faculty of Medicine, Suleyman Demirel University, Isparta, Turkey

    Ayşe Bütün & Serpil Demirci

  2. Department of Biophysics, Faculty of Medicine, Suleyman Demirel University, Isparta, Turkey

    Mustafa Nazıroğlu, Ömer Çelik & Abdulhadi Cihangir Uğuz

  3. Neuroscience Research Center, Suleyman Demirel University, 32260, Isparta, Turkey

    Mustafa Nazıroğlu, Serpil Demirci, Ömer Çelik & Abdulhadi Cihangir Uğuz

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  1. Ayşe Bütün
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Correspondence to Mustafa Nazıroğlu.

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Bütün, A., Nazıroğlu, M., Demirci, S. et al. Riboflavin and Vitamin E Increase Brain Calcium and Antioxidants, and Microsomal Calcium-ATP-ase Values in Rat Headache Models Induced by Glyceryl Trinitrate. J Membrane Biol 248, 205–213 (2015). https://doi.org/10.1007/s00232-014-9758-5

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