Abstract
Zinc is one of the required trace elements in animals, and it serves an important role in biological systems. However, high levels of zinc are poisonous to organisms. So far, there exist conflicting reports about zinc ions-induced mitochondrial permeability transition (MPT). We analyzed the effects of Zn2+ on MPT by monitoring mitochondrial swelling with the ultraviolet–visible light absorption spectrum, characterizing the fluidity of the membrane with fluorescence anisotropy, detecting the transmembrane potential (Δψ) with fluorescence intensity, and observing mitochondrial ultrastructure with transmission electron microscopy. Data reveal that low concentrations of zinc ions can trigger MPT while high levels of zinc ions cannot, which implies that zinc ions’ toxicity cannot be the result of a single simple mechanism.
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Acknowledgments
The authors gratefully acknowledge the financial support of Chinese 973 program (grant 2011CB933600), and National Natural Science Foundation of China (grant 21077081, 21173026, and 20921062), and Fundamental Research Funds for Central Universities (1103005, 1101007).
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Liu, XR., Li, JH., Zhang, Y. et al. Mitochondrial Permeability Transition Induced by Different Concentrations of Zinc. J Membrane Biol 244, 105–112 (2011). https://doi.org/10.1007/s00232-011-9403-5
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DOI: https://doi.org/10.1007/s00232-011-9403-5