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Development and application of highly specific PCR for detection of chicken (Gallus gallus) meat adulteration

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Abstract

In order to prevent fraud in the sale and strengthen quality assurance, authentic identification of chicken meat is essential. In the present investigation, a chicken (Gallus gallus)-specific polymerase chain reaction (PCR) was developed for the unambiguous identification of chicken meat. The PCR assay employs pair of primers designed against chicken nuclear 5-aminolevulinate (ALA) synthase gene. Highly chicken-specific diagnostic amplicon of 288 bp was established upon PCR and was evident in all the nine breeds/strains of chicken species. Sensitivity of PCR in detecting chicken meat adulteration was established to be at 0.1 % in the foreign meat matrix, while limit of detection (LOD) of chicken DNA was 10 pg. Suitability of the developed chicken-specific PCR was validated and confirmed in raw, cooked/heat treated (60, 80, 100, and 121 °C), and micro-oven cooked meat samples. Possibility of cross-amplification of adulterating DNA was excluded by cross-checking the developed PCR assay with several animal and avian species. The PCR assay developed in this study is highly promising for applications involving circumstances that require authentic identification of chicken meat.

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Acknowledgments

The authors thank i. the Department of Biotechnology (DBT), Government of India, New Delhi, ii. The Dean, College of Veterinary and Animal Sciences, G.B.P.U.A.T., Pantnagar, and iii. The officials of High Altitude Zoo, Nainital (Uttarakhand, India) for their support rendered in accomplishing this work.

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Authors and Affiliations

  1. Department of Veterinary Public Health and Epidemiology, Veterinary College, Shimoga, Karnataka, 577 204, India

    Nagappa S. Karabasanavar

  2. Department of Veterinary Public Health, College of Veterinary and Animal Sciences, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263145, India

    S. P. Singh

  3. Department of Veterinary Public Health and Epidemiology, College of Veterinary Science and Animal Husbandry, R. K. Nagar, Agartala, Tripura (West), 799008, India

    Deepak Kumar

  4. Gennova Biopharmaceutical, Pune, Maharashtra, India

    Sunil N. Shebannavar

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  1. Nagappa S. Karabasanavar
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  2. S. P. Singh
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  4. Sunil N. Shebannavar
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Correspondence to Nagappa S. Karabasanavar.

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Karabasanavar, N.S., Singh, S.P., Kumar, D. et al. Development and application of highly specific PCR for detection of chicken (Gallus gallus) meat adulteration. Eur Food Res Technol 236, 129–134 (2013). https://doi.org/10.1007/s00217-012-1868-7

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  • DOI: https://doi.org/10.1007/s00217-012-1868-7

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