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Effect of ionic liquid-containing system on betulinic acid production from betulin biotransformation by cultured Armillaria luteo-virens Sacc cells

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Abstract

The aim of this study is to develop an ionic liquid-containing system for efficient production of betulinic acid by cultured cells Armillaria luteo-virens Sacc ZJUQH100-6. Several parameters affecting betulinic acid formation in the IL-containing system were investigated. The addition of [EMIM][BF4] in hexane-containing reaction medium gave rise to better betulinic acid formation in comparison with other ILs used. The optimal concentration of IL in IL-containing co-solvent system is 50% (v/v). As a co-substrate, butanol is found to be useful for intracellular betulin-28-monooxygenase synthesis during the whole phase. The concentrations of substrate and resting cells have been found to exert a significant effect on betulinic acid. Moreover, the reaction time in this IL-containing system was less than that in the conventional one. The effect of the constructed IL-containing system on cell membrane structure was comparatively observed. Under the developed IL-containing system, the highest yield of product observed was 11.14% at 18 h, higher than that in monophase aqueous one (P < 0.05), whereas the activity of monooxygenase showed the same variation as betulinic acid formation.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (No. 20806069).

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Authors and Affiliations

  1. Department of Food Science and Nutrition, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Fu Ming-liang, Liu **g, Dong Ya-chen, Feng Yu, Fang Ruo-si, Chen Qi-he & Liu **ao-jie

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  1. Fu Ming-liang
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  2. Liu **g
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  3. Dong Ya-chen
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Correspondence to Chen Qi-he or Liu **ao-jie.

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Ming-liang, F., **g, L., Ya-chen, D. et al. Effect of ionic liquid-containing system on betulinic acid production from betulin biotransformation by cultured Armillaria luteo-virens Sacc cells. Eur Food Res Technol 233, 507–515 (2011). https://doi.org/10.1007/s00217-011-1549-y

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  • DOI: https://doi.org/10.1007/s00217-011-1549-y

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