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A near-infrared turn-on fluorescence probe for glutathione detection based on nanocomposites of semiconducting polymer dots and MnO2 nanosheets

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Abstract

Fluorescence biosensors that enable highly sensitive detection of glutathione (GSH) are in great demand for various biological investigations and early disease diagnoses. Here, we report a turn-on fluorescence nanoplatform based on fluorescent semiconducting polymer nanoparticle@MnO2 nanosheets (P-dot@MnO2) nanocomposites for rapid homogeneous determination of GSH. The near-infrared (NIR) fluorescent P-dots were prepared by do** NIR dyes into polymer matrix and then encompassed by MnO2, which resulted in a remarkable fluorescence quenching. Owing to the selective decomposition of MnO2 by GSH, the fluorescence recovery was achieved in the presence of GSH. On the basis of the target-induced turn-on fluorescence response, the developed nanoplatform can readily detect GSH with a high sensitivity up to 0.26 μM, as well as a superior specificity. Furthermore, it was successfully applied in monitoring the intracellular GSH in living cells, revealing its great potential in biomedical applications.

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Funding

This work was supported by the National Natural Science Foundation of China (Grant No. 21605021), the China Postdoctoral Science Foundation (Grant No. 2019M652241), the Young and Middle-Aged Talent Training Project of Fujian Provincial Health and Family Planning Commission (Grant No. 2018-ZQN-75), the Medical Innovation grant of Fujian Province (Grant No. 2018-CX-49), and the Startup Fund of Mengchao Hepatobiliary Hospital of Fujian Medical University (Grant No. QDZJ-2017-004).

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Authors and Affiliations

  1. College of Chemistry, Fuzhou University, Fuzhou, 350116, Fujian, China

    Cheng Zheng

  2. College of Biological Science and Engineering, Fuzhou University, Fuzhou, 350116, Fujian, China

    Lei Ding, Yanni Wu, Yongyi Zeng, **aolong Zhang, **aolong Liu & **gfeng Liu

  3. The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, Fujian, China

    Lei Ding, Yanni Wu, **onghong Tan, Yongyi Zeng, **aolong Zhang, **aolong Liu & **gfeng Liu

  4. Liver Disease Center, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, Fujian, China

    Yongyi Zeng & **gfeng Liu

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  1. Cheng Zheng
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  2. Lei Ding
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Correspondence to **aolong Zhang or **gfeng Liu.

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Zheng, C., Ding, L., Wu, Y. et al. A near-infrared turn-on fluorescence probe for glutathione detection based on nanocomposites of semiconducting polymer dots and MnO2 nanosheets. Anal Bioanal Chem 412, 8167–8176 (2020). https://doi.org/10.1007/s00216-020-02951-8

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