Abstract
Acute myocardial infarction (AMI) is one of the leading risks to global health. Thus, the rapid, accurate early diagnosis of AMI is highly critical. Human cardiac troponin I (cTnI) has been regarded as a golden biomarker for AMI due to its excellent selectivity. In this work, a novel fluorescent aptasensor based on a graphene oxide (GO) platform was developed for the highly sensitive and selective detection of cTnI. GO binds to the fluorescent anti-cTnI aptamer and quenches its fluorescence. In the presence of cTnI, the fluorescent anti-cTnI aptamer leaves the surface of GO, combines with cTnI because of the powerful affinity of the fluorescent anti-cTnI aptamer and cTnI, and then restores the fluorescence of the fluorescent anti-cTnI aptamer. Fluorescence-enhanced detection is highly sensitive and selective to cTnI. The method exhibited good analytical performance with a reasonable dynamic linearity at the concentration range of 0.10–6.0 ng/mL and a low detection limit of 0.07 ng/mL (S/N = 3). The fluorescent aptasensor also exhibited high selectivity toward cTnI compared with other interference proteins. The proposed method may be a potentially useful tool for cTnI determination in human serum.
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Funding
This work was supported by the National Natural Science Foundation of China (Nos. 21677060 and 51503079), the Zhejiang Provincial Natural Science Foundation of China under Grant No. LY16B050007, the Public Welfare Research Project of Zhejiang Province (No. LGF18B050004), and the Science and Technology Plan Project of Jiaxing City, China (No. 2017AY33034).
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Human serum sample used in this study was collected from a healthy volunteer. This work was performed with the written informed consent of the healthy volunteer. The studies were approved by the Medical Ethics Committee of **n’an International Hospital (Jiaxing, China) and performed in accordance with the ethical standards.
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The authors declare that they have no conflict of interest.
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Liu, D., Lu, X., Yang, Y. et al. A novel fluorescent aptasensor for the highly sensitive and selective detection of cardiac troponin I based on a graphene oxide platform. Anal Bioanal Chem 410, 4285–4291 (2018). https://doi.org/10.1007/s00216-018-1076-9
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DOI: https://doi.org/10.1007/s00216-018-1076-9