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A multi-residue method for the determination of seven polypeptide drug residues in chicken muscle tissues by LC-MS/MS

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Abstract

A new multi-residue method for the determination of seven polypeptides, namely, polymixin B1, polymixin B2, polymixin E1 (colistin A), polymixin E2 (colistin B), enduracidin A (enramycin A), enduracidin B (enramycin B), and bacitracin A, in food of animal origin was developed and validated for chicken muscle tissue. Chicken muscle tissue was extracted with acidified methanol (1 % TFA). After homogenization, shaking, and centrifugation, the acidified methanol extract was decanted. A second extraction was performed with methanol (1 % TFA) and formic acid (1 %) 25:75, v/v. The pooled extract was cleaned up and concentrated on a solid-phase extraction cartridge. The retained analytes were eluted with methanol/acetonitrile. The extract was evaporated to dryness, reconstituted in mobile phase, filtered, and quantified by LC-MS/MS under ESI conditions. The method has a LOQ of 50.0 μg/kg for polymixin E2 (colistin B), 39.0 μg/kg for polymixin E1 (colistin A), 74.0 μg/kg for polymixin B1, 71.0 μg/kg for polymixin B2, 66.0 μg/kg for enduracidin A, 50.0 μg/kg for enduracidin B, and 30.0 μg/kg for bacitracin A in chicken muscle tissues. This is the first sensitive, suitable, multi-residue method reported for the seven polypeptide drug residues in chicken muscle tissue.

Effect of chicken muscle tissue on the ESI +ve detection signal for Colistin A showing signal suppression in the presence of matrix

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References

  1. Council Regulation 2821/98, Commission regulation 2788/98 (1998) Off J Eur Commun Legis L347:32

    Google Scholar 

  2. Commission of the European Communities, Council Directive 70/524/EEC (1994) Off J Eur Commun Legis L270:1

    Google Scholar 

  3. Lee S, Gedir R, Boison JO (2009) A determinative and confirmatory method for residues of the metabolites of carbadox and olaquindox in porcine tissues. Anal Chim Acta 637:128–134

    Article  Google Scholar 

  4. Lee S, Matus J, Gedir R, Boison J (2011) A validated LC-MS method for the determination of bacitracin drug residues in edible pork tissues with confirmation by LC-tandem mass spectrometry. J Liq Chromatogr Relat Technol 34:2699–2722

    Article  CAS  Google Scholar 

  5. Boison J, Lee S, Gedir R (2009) Analytical determination of virginiamycin drug residues in edible porcine tissues by LC-MS with confirmation by LC-MS/MS. J AOAC Int 92:329–339

    CAS  Google Scholar 

  6. Dickson LC, O’Byrne C, Chan W (2012) A quantitative method for residues of macrolide antibiotics in porcine kidney by liquid chromatography-tandem mass spectrometry. J AOAC Int 95(2):567–575

    Article  CAS  Google Scholar 

  7. Ikai Y (1995) Chemical analysis of peptide antibiotics. In: Oka H, Nakazawa H, Harada K, MacNeil JD (eds) Chemical analysis of antibiotics used in agriculture, 1st edn. AOAC International, Arlington, VA, USA, pp 407–437, 22201–3301

    Google Scholar 

  8. Horie M, Hoshino Y, Nose N, Nakazawa H, Fujita M, Takabatake E (1985) Determination of enramycin in chicken and swine muscles by high performance liquid chromatography. Food Hyg Saf Sci Shokuhin Eiseigaku Zasshi Jpn 26(4):337–342

    Article  CAS  Google Scholar 

  9. Nagase M, Fukamachi K (1987) Determination of virginiamycin in swine, cattle and chicken muscle by HPLC with fluorescence detection. Bunseki Kagaku 36:297–300

    Article  CAS  Google Scholar 

  10. Moats WA, Leskinen L (1988) Determination of virginiamycin residues in swine tissue using high performance liquid chromatography. J Agric Food Chem 36:1297–1300

    Article  CAS  Google Scholar 

  11. Saito K, Horie M, Hoshino Y, Nose N, Nakazawa H, Fujita M (1989) Determination of virginiamycin in chicken and swine tissues by high performance liquid chromatography. Eisei Kagaku 35:63–67

    Article  CAS  Google Scholar 

  12. Decolin D, Leroy P, Nicolas A, Archimbault P (1997) Hyphenated liquid chromatographic method for the determination of colistin residues in bovine tissues. J Chromatogr Sci 35:557–564

    Article  CAS  Google Scholar 

  13. Wan EC-H, Sin DW-M, Wong Y-C (2006) Detection of residual bacitracin A, colistin A and colistin B in milk and animal tissues by liquid chromatography tandem mass spectrometry. Anal Bioanal Chem 385:181–188

    Article  CAS  Google Scholar 

  14. Kaufman A, Widmer M (2013) Quantitative analysis of polypeptide antibiotic residues in a variety of food matrices by liquid chromatography coupled to tandem mass spectrometry. Anal Chim Acta 797:81–88

    Article  Google Scholar 

  15. Sin DWM, Ho C, Wong Y-C, Ho S-K, Ip AC-B (2005) Analysis of major components of residual bacitracin and colistin in food samples by liquid chromatography tandem mass spectrometry. Anal Chim Acta 535:23–31

    Article  CAS  Google Scholar 

  16. Matuszewski BK (2006) Standard line slopes as a measure of a relative matrix effect in quantitative HPLC-MS bioanalysis. J Chromatogr B 830:293–300

    Article  CAS  Google Scholar 

Download references

Acknowledgment

The authors acknowledge Jana Kinar of the Canadian Food Inspection Agency (CFIA) Laboratory in Saskatoon for technical assistance in the laboratory.

Conflict of interest

Mention of a company name or brand name does not constitute an endorsement by the Canadian Food Inspection Agency Laboratory in Saskatoon above others of a similar nature or product.

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Authors and Affiliations

  1. Centre for Veterinary Drug Residues, Canadian Food Inspection Agency (CVDR), Saskatoon Laboratory, 116 Veterinary Road, Saskatoon, Saskatchewan, S7N 2R3, Canada

    Joe O. Boison, Stephen Lee & Johanna Matus

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  1. Joe O. Boison
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  2. Stephen Lee
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  3. Johanna Matus
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Correspondence to Joe O. Boison.

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Boison, J.O., Lee, S. & Matus, J. A multi-residue method for the determination of seven polypeptide drug residues in chicken muscle tissues by LC-MS/MS. Anal Bioanal Chem 407, 4065–4078 (2015). https://doi.org/10.1007/s00216-015-8644-z

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