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Development of a colloidal gold-based lateral-flow immunoassay for the rapid simultaneous detection of clenbuterol and ractopamine in swine urine

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Abstract

A multianalyte lateral-flow immunochromatographic technique using colloidal gold-labeled polyclonal antibodies was developed for the rapid simultaneous detection of clenbuterol and ractopamine. The assay procedure could be accomplished within 5 min, and the results of this qualitative one-step assay were evaluated visually according to whether test lines appeared or not. When applied to the swine urines, the detection limit and the half maximal inhibitory concentration (IC50) of the test strip under an optical density scanner were calculated to be 0.1 ± 0.01 ng mL−1 and 0.1 ± 0.01 ng mL−1, 0.56 ± 0.08 ng mL−1, and 0.71 ± 0.06 ng mL−1, respectively, the cut-off levels with the naked eye of 1 ng mL−1 and 1 ng mL−1 for clenbuterol and ractopamine were observed. Parallel analysis of swine urine samples with clenbuterol and ractopamine showed comparable results obtained from the multianalyte lateral-flow test strip and GC-MS. Therefore, the described multianalyte lateral-flow test strip can be used as a reliable, rapid, and cost-effective on-site screening technique for the simultaneous determination of clenbuterol and ractopamine residues in swine urine.

The colloidal gold-based lateral-flow immunoassay for the rapid simultaneous detection of clenbuterol and ractopamine in swine urine.

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Acknowledgment

This work was supported by the Important Science and Technology Specific Program of Zhejiang Province (Grant No. 2006C12102) and the Innovation Fund for Technology-based Firms from the Ministry of Science and Technology of China (Grant No. 08C26213300756).

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Authors and Affiliations

  1. Institute of Bio-safety and Food Science, College of Life Science, China Jiliang University, Hangzhou, 310018, People’s Republic of China

    Ming-Zhou Zhang, Zong-Lun Chen, Jie-Hong Fang, Mei-Ming Fang, Jun Liu & **ao-** Yu

  2. Hangzhou DNA Sci-tech Co., Ltd., Hangzhou, 310023, People’s Republic of China

    Min-Zi Wang & Zong-Lun Chen

  3. **asha Higher Education Zone, Xueyuan Street, Hangzhou, 310018, People’s Republic of China

    Ming-Zhou Zhang

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  1. Ming-Zhou Zhang
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  2. Min-Zi Wang
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  3. Zong-Lun Chen
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  5. Mei-Ming Fang
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  7. **ao-** Yu
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Correspondence to Ming-Zhou Zhang.

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Zhang, MZ., Wang, MZ., Chen, ZL. et al. Development of a colloidal gold-based lateral-flow immunoassay for the rapid simultaneous detection of clenbuterol and ractopamine in swine urine. Anal Bioanal Chem 395, 2591–2599 (2009). https://doi.org/10.1007/s00216-009-3181-2

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  • DOI: https://doi.org/10.1007/s00216-009-3181-2

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