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Fungal wood-degrading enzymes in esca-diseased grapevine and effects of carbohydrate environment on fungal development

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Abstract

In esca disease affecting grapevines, Phaeomoniella chlamydospora and Phaeoacremonium minimum colonize the woody parts of the trunks and arms, where they obtain nutrition from xylem sap and, potentially, from residues resulting from the enzymatic breakdown of lignified cell walls, particularly osidic residues. We quantified the secretion of lignin peroxidase, manganese peroxidase and laccase by these fungi in woody tissues of selectively infected cuttings using immunolabeling and transmission electron microscopy. Our results indicated that the detection of these enzymes was generally higher in tissues infected with Phaeoacremonium minimum. These data were confirmed through immunodetection of enzymes secreted by hyphae of fungi grown in vitro. Additionally, we observed that the supply of various carbohydrates (mono, di, tri and tetrasaccharides and polymers) differentially influenced fungal growth and polypeptide secretion. Since some secreted polypeptides display detrimental effects on grapevine cells, these results raise the question of whether the carbohydrate environment could be a factor affecting the aggressiveness of these pathogens.

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Data availability

All datasets are presented in the main manuscript and supplementary data S1 and S2.

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Acknowledgements

The authors are grateful to Emile Béré and Bruno Merceron for their assistance in the Image UP Service (Microscopie Electronique et Photonique, Bâtiment B36, TSA 51106, 86022 Poitiers); They also would thank Raphaël Decou, Laboratoire PEIRENE, EA 7500, 123, avenue Albert Thomas, 87060 Limoges for his contribution in wall components analysis.

Funding

Part of the work was financially supported by the Bureau National Interprofessionnel du Cognac (Ph D of EL). The work received additional support from the following 2015–2020 programs: the State-Region Planning Contracts (CPER) and the European Regional Development Fund (FEDER).

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  1. Laboratoire EBI (Ecologie et Biologie des Interactions), UMR CNRS 7267, Université de Poitiers, 3 rue Jacques Fort, TSA 51106, 86073, Poitiers Cedex 9, France

    Pierrette Fleurat-Lessard, Estelle Luini, Sylvain La Camera, Florence Thibault, Gabriel Roblin & Jean-Marc Berjeaud

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  1. Pierrette Fleurat-Lessard
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  2. Estelle Luini
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  3. Sylvain La Camera
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Contributions

The work was conceived by JMB and GR. Experiments were performed by EL, FT and PFL. SLC, PFL, JMB and GR contributed to the analysis and interpretation of the data. The manuscript was written through contributions of all authors.

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Correspondence to Jean-Marc Berjeaud.

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Communicated by Yusuf Akhter.

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Fleurat-Lessard, P., Luini, E., La Camera, S. et al. Fungal wood-degrading enzymes in esca-diseased grapevine and effects of carbohydrate environment on fungal development. Arch Microbiol 205, 194 (2023). https://doi.org/10.1007/s00203-023-03544-6

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