Abstract
A polyphasic study was conducted with 11 strains trapped by Mimosa pudica and Phaseolus vulgaris grown in soils of the Brazilian Atlantic Forest. In the phylogenetic analysis of the 16S rRNA gene, one clade of strains (Psp1) showed higher similarity with Paraburkholderia piptadeniae STM7183T (99.6%), whereas the second (Psp6) was closely related to Paraburkholderia tuberum STM678T (99%). An MLSA (multilocus sequence analysis) with four (recA, gyrB, trpB and gltB) housekee** genes placed both Psp1 and Psp6 strains in new clades, and BOX-PCR profiles indicated high intraspecific genetic diversity within each clade. Values of digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) of the whole genome sequences were of 56.9 and 94.4% between the Psp1 strain CNPSo 3157T and P. piptadeniae; and of 49.7% and 92.7% between the Psp6 strain CNPSo 3155T and P. tuberum, below the threshold for species delimitation. In the nodC analysis, Psp1 strains clustered together with P. piptadeniae, while Psp6 did not group with any symbiotic Paraburkholderia. Other phenotypic, genotypic and symbiotic properties were evaluated. The polyphasic analysis supports that the strains represent two novel species, for which the names Paraburkholderia franconis sp. nov. with type strain CNPSo 3157T (= ABIP 241, = LMG 31644) and Paraburkholderia atlantica sp. nov. with type strain CNPSo 3155T (= ABIP 236, = LMG 31643) are proposed.
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21 May 2021
A Correction to this paper has been published: https://doi.org/10.1007/s00203-021-02373-9
Abbreviations
- NI:
-
Nucleotide identity
- ANI:
-
Average nucleotide identity
- BNF:
-
Biological nitrogen fixation
- DDH:
-
DNA–DNA hybridization
- dDDH:
-
Digital DNA–DNA hybridization
- MLSA:
-
Multilocus sequence analysis
- PCR:
-
Polymerase chain reaction
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Acknowledgements
F. Paulish acknowledges a PhD fellowship from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil—Finance Code 001)-Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná, R. F. Dall’Agnol a posdoc fellowship from CAPES-Embrapa and J. R. M. Delamuta and R.A. Ribeiro fellowships from CNPq (Brazilian National Research Council). The authors acknowledge Ligia Maria O. Chueire (Embrapa Soja) and Dr. Sergio Miana for help in several steps of this study, and Dr. Aharon Oren for help in the epithet proposal.
Funding
Funded by INCT-Plant-Growth Promoting Microorganisms for Agricultural Sustainability and Environmental Responsibility (CNPq 465133/2014–2, Fundação Araucária-STI 043/2019, CAPES), Embrapa (02.13.08.001.00.00), CNPq-Universal (400468/2016–6).
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Paulitsch, F., Dall’Agnol, R.F., Delamuta, J.R.M. et al. Paraburkholderia atlantica sp. nov. and Paraburkholderia franconis sp. nov., two new nitrogen-fixing nodulating species isolated from Atlantic forest soils in Brazil. Arch Microbiol 202, 1369–1380 (2020). https://doi.org/10.1007/s00203-020-01843-w
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DOI: https://doi.org/10.1007/s00203-020-01843-w